http://hdl.handle.net/10171/196 2013-05-23T00:03:34Z 2013-05-23T00:03:34Z Unzu, C. (Carmen) Hervas-Stubbs, S. (Sandra) Sampedro, A. (Ana) Mauleon, I. (Itsaso) Mancheño, U. (Uxua) Alfaro, C. (Carlos) Salamanca, E. (Enrique) de Benito, A. (Alberto) Beatties, S.G. (Stuart G.) Pety, H. (Harald) Prieto, J. (Jesús) Melero, I. (Ignacio) Fontanellas, A. (Antonio) http://hdl.handle.net/10171/23057 2012-08-14T00:10:01Z 2011-12-31T23:00:00Z

Title: Transient and intensive pharmacological immunosuppression fails to improve AAV-based liver gene transfer in non-human primates Author(s) : Unzu, C. (Carmen); Hervas-Stubbs, S. (Sandra); Sampedro, A. (Ana); Mauleon, I. (Itsaso); Mancheño, U. (Uxua); Alfaro, C. (Carlos); Salamanca, E. (Enrique) de; Benito, A. (Alberto); Beatties, S.G. (Stuart G.); Pety, H. (Harald); Prieto, J. (Jesús); Melero, I. (Ignacio); Fontanellas, A. (Antonio) Abstract: BACKGROUND: Adeno-associated vectors (rAAV) have been used to attain long-term liver gene expression. In humans, the cellular immune response poses a serious obstacle for transgene persistence while neutralizing humoral immunity curtails re-administration. Porphobilinogen deaminase (PBGD) haploinsufficiency (acute intermittent porphyria) benefits from liver gene transfer in mouse models and clinical trials are about to begin. In this study, we sought to study in non-human primates the feasibility of repeated gene-transfer with intravenous administration of rAAV5 vectors under the effects of an intensive immunosuppressive regimen and to analyze its ability to circumvent T-cell immunity and thereby prolong transgene expression. METHODS: Three female Macaca fascicularis were intravenously injected with 1x1013 genome copies/kg of rAAV5 encoding the human PBGD. Mycophenolate mofetil (MMF), anti-thymocyte immunoglobulin, methylprednisolone, tacrolimus and rituximab were given in combination during 12 weeks to block T- and B-cell mediated adaptive immune responses in two macaques. Immunodeficient and immunocompetent mice were intravenously injected with 5x1012 genome copies/kg of rAAV5-encoding luciferase protein. Forty days later MMF, tacrolimus and rituximab were daily administrated to ascertain whether the immunosuppressants or their metabolites could interfere with transgene expression. RESULTS: Macaques given a rAAV5 vector encoding human PBGD developed cellular and humoral immunity against viral capsids but not towards the transgene. Anti-AAV humoral responses were attenuated during 12 weeks but intensely rebounded following cessation of the immunosuppressants. Accordingly, subsequent gene transfer with a rAAV5 vector encoding green fluorescent protein was impossible. One macaque showed enhanced PBGD expression 25 weeks after rAAV5-pbgd administration but overexpression had not been detected while the animal was under immunosuppression. As a potential explanation, MMF decreases transgene expression in mouse livers that had been successfully transduced by a rAAV5 several weeks before MMF onset. Such a silencing effect was independent of AAV complementary strand synthesis and requires an adaptive immune system. CONCLUSIONS: These results indicate that our transient and intensive pharmacological immunosuppression fails to improve AAV5-based liver gene transfer in non-human primates. The reasons include an incomplete restraint of humoral immune responses to viral capsids that interfere with repeated gene transfer in addition to an intriguing MMF-dependent drug-mediated interference with liver transgene expression.

2011-12-31T23:00:00Z Seijo, L. (Luis) Torres, J.P. (Juan P.) de Lozano, M.D. (María Dolores) Bastarrika, G. (Gorka) Alcaide, A.B. (Ana Belén) Lacunza, M.M. (María M.) Zulueta, J.J. (Javier J.) http://hdl.handle.net/10171/22930 2012-10-03T08:45:44Z 2009-12-31T23:00:00Z

Title: Diagnostic yield of electromagnetic navigation bronchoscopy is highly dependent on the presence of a Bronchus sign on CT imaging: results from a prospective study Author(s) : Seijo, L. (Luis); Torres, J.P. (Juan P.) de; Lozano, M.D. (María Dolores); Bastarrika, G. (Gorka); Alcaide, A.B. (Ana Belén); Lacunza, M.M. (María M.); Zulueta, J.J. (Javier J.) Abstract: Electromagnetic navigation bronchoscopy (ENB) has been developed as a novel ancillary tool for the bronchoscopic diagnosis of pulmonary nodules. Despite successful navigation in 90% of patients, ENB diagnostic yield does not generally exceed 70%. We sought to determine whether the presence of a bronchus sign on CT imaging conditions diagnostic yield of ENB and might account for the discrepancy between successful navigation and diagnostic yield. METHODS: We conducted a prospective, single-center study of ENB in 51 consecutive patients with pulmonary nodules. ENB was chosen as the least invasive diagnostic technique in patients with a high surgical risk, suspected metastatic disease, or advanced-stage disease, or in those who demanded a preoperative diagnosis prior to undergoing curative resection. We studied patient and technical variables that might condition diagnostic yield, including size, cause, location, distance to the pleural surface, and fluorodeoxyglucose uptake of a given nodule; the presence of a bronchus sign on CT imaging; registration point divergence; and the minimum distance from the tip of the locatable guide to the nodule measured during the procedure. RESULTS: The diagnostic yield of ENB was 67% (34/51). The sensitivity and specificity of ENB for malignancy in this study were 71% and 100%, respectively. ENB was diagnostic in 79% (30/38) patients with a bronchus sign on CT imaging but only in 4/13 (31%) with no discernible bronchus sign. Univariate analysis identified the bronchus sign (P = .005) and nodule size (P = .04) as statistically significant variables conditioning yield, but on multivariate analysis, only the bronchus sign remained significant (OR, 7.6; 95% CI, 1.8-31.7). No procedure-related complications were observed. CONCLUSIONS: ENB diagnostic yield is highly dependent on the presence of a bronchus sign on CT imaging.

2009-12-31T23:00:00Z Bastarrika, G. (Gorka) Cecco, C.N. (C.N.) de Anselmi, A. (Amedeo) Herreros, J. (Jesús) http://hdl.handle.net/10171/22805 2012-07-04T00:08:39Z 2007-12-31T23:00:00Z

Title: Incidental dual source computed tomography imaging of ductal aortic coarctation, left subclavian artery stenosis and bicuspid aortic valve in a patient admitted for atypical chest pain Author(s) : Bastarrika, G. (Gorka); Cecco, C.N. (C.N.) de; Anselmi, A. (Amedeo); Herreros, J. (Jesús) Abstract: A case of incidental ductal aortic coarctation with left subclavian artery stenosis at the origin, severely calcified and stenotic bicuspid aortic valve, and normal coronary arteries demonstrated by single breath hold dual source computed tomography angiography in a 46-year-old man admitted for acute chest pain is presented.

2007-12-31T23:00:00Z Bastarrika, G. (Gorka) Cecco, C.N. (C.N.) de Arraiza, M. (María) Mastrobuoni, S. (Stefano) Pueyo, J. (Jesús) Ubilla, M. (Matias) Rabago, G. (Gregorio) http://hdl.handle.net/10171/22692 2013-01-08T16:16:38Z 2007-12-31T23:00:00Z

Title: Dual-source CT for visualization of the coronary arteries in heart transplant patients with high heart rates Author(s) : Bastarrika, G. (Gorka); Cecco, C.N. (C.N.) de; Arraiza, M. (María); Mastrobuoni, S. (Stefano); Pueyo, J. (Jesús); Ubilla, M. (Matias); Rabago, G. (Gregorio) Abstract: OBJECTIVE. The purpose of this study was to evaluate the quality of dual-source CT images of the coronary arteries in heart transplant recipients with high heart rates. SUBJECTS AND METHODS. Contrast-enhanced dual-source CT coronary angiography was performed on 23 heart transplant recipients (20 men, three women; mean age, 61.1 ± 12.8 years). Data sets were reconstructed in 5% steps from 30% to 80% of the R-R interval. Two blinded independent readers using a 5-point scale (0, not evaluative; 4, excellent quality) assessed the quality of images of coronary segments. RESULTS. The mean heart rate during scanning was 89.2 ± 10.4 beats/min. Interobserver agreement on the quality of images of the whole coronary tree was a kappa value of 0.78 and for selection of the optimal reconstruction interval was a kappa value of 0.82. The optimal reconstruction interval was systole in 17 (74%) of the 23 of heart transplant recipients. At the best reconstruction interval, diagnostic image quality (score ≥ 2) was obtained in 92.1% (303 of 329) of the coronary artery segments. The mean image quality score for the whole coronary tree was 3.1 ± 1.01. No significant correlation between mean heart rate (ρ = 0.31) or heart rate variability (ρ = 0.23) and overall image quality score was observed (p = not significant). CONCLUSION. Dual-source CT acquisition yields coronary angiograms of diagnostic quality in heart transplant recipients. Mean heart rate and heart rate variability during scanning do not have a negative effect on the overall quality of images of the coronary arteries.

2007-12-31T23:00:00Z Bastarrika, G. (Gorka) Azcarate, P.M. (Pedro María) Arias, J. (J.) Pueyo, J. (Jesús) Castaño, S. (S.) Gavira, J.J. (Juan José) http://hdl.handle.net/10171/22581 2013-01-08T16:17:08Z 2008-12-31T23:00:00Z

Title: Miocarditis aguda: diagnóstico mediante resonancia magnética cardiaca Author(s) : Bastarrika, G. (Gorka); Azcarate, P.M. (Pedro María); Arias, J. (J.); Pueyo, J. (Jesús); Castaño, S. (S.); Gavira, J.J. (Juan José) Abstract: Cardiomyopathies are a common cause of morbidity and mortality. Myocarditis, which is included among specific cardiomyopathies, frequently presents non-specific clinical manifestations and thus may be difficult to diagnose, or even be misdiagnosed. Traditionally employed diagnostic techniques, including endomyocardial biopsy, have been shown to be of limited value. Following its overall implantation in clinical practice, cardiac magnetic resonance imaging (MRI) is nowadays widely considered to be the best non-invasive diagnostic tool available for diagnosing myocarditis

2008-12-31T23:00:00Z Bastarrika, G. (Gorka) Cecco, C.N. (C.N.) de Mastrobuoni, S. (Stefano) Arraiza, M. (M.) Pueyo, J. (Jesús) Rabago, G. (Gregorio) http://hdl.handle.net/10171/22540 2013-01-08T16:17:37Z 2008-12-31T23:00:00Z

Title: Valoración global del corazón en el paciente con transplante cardiaco mediante tomografía computarizada de doble fuente Author(s) : Bastarrika, G. (Gorka); Cecco, C.N. (C.N.) de; Mastrobuoni, S. (Stefano); Arraiza, M. (M.); Pueyo, J. (Jesús); Rabago, G. (Gregorio) Abstract: In routine clinical practice surveillance of heart transplant recipients is usually performed using echocardiography and conventional coronary angiography. The latter permits diagnosis and follow-up of coronary allograft vasculopathy. However, this procedure is invasive and is not free of complications. Conventional multislice computed tomography (MSCT) has been shown to be a useful non-invasive tool for ruling out coronary artery disease and evaluating cardiac function. However, due to its limited temporal resolution betablocker administration is required, and its usefulness in certain patient populations with restricted response to this medication, such as heart transplant recipients, may therefore be limited. Dual-source CT (DSCT) allows evaluation of the coronary arteries in all individuals independent of their heart rate. In the case presented here, we demonstrate that DSCT may be useful for evaluating cardiac function and ruling out coronary allograft vasculopathy in heart transplant recipients.

2008-12-31T23:00:00Z Hernandez, C. (C.) Zudaire, B. (B.) Castaño, S. (S.) Azcarate, P.M. (Pedro María) Villanueva, A. (Alberto) Bastarrika, G. (Gorka) http://hdl.handle.net/10171/22529 2012-10-09T11:43:51Z 2006-12-31T23:00:00Z

Title: Principios básicos de resonancia magnética cardiovascular (RMC): secuencias, planos de adquisición y protocolo de estudio Author(s) : Hernandez, C. (C.); Zudaire, B. (B.); Castaño, S. (S.); Azcarate, P.M. (Pedro María); Villanueva, A. (Alberto); Bastarrika, G. (Gorka) Abstract: ABSTRACT Evaluation of the cardiovascular system with magnetic resonance (CMR) has become one of the most relevant and up-to-the-minute clinical applications of this diagnostic technique, as CMR makes possible an exact and reproducible study of the anatomy and function of the heart and great vessels. The complexity of this technique is mainly due to the anatomical location and orientation of the cardiovascular structures, the specific CMR sequences that have to be used and a lack of familiarity amongst radiologists regarding cardiovascular pathology. In this report the most basic principles of CMR are described. The clinical usefulness of anatomical, functional, and flow quantification sequences are discussed, conventional CMR acquisition planes are described, and an easy CMR study protocol is proposed.

2006-12-31T23:00:00Z Villanueva, A. (Alberto) Caceres, J. (José) Ferreira, M. (Mónica) Brocano, J. (Jordi) Pallisa, E. (Esther) Bastarrika, G. (Gorka) http://hdl.handle.net/10171/22517 2012-06-09T00:08:43Z 2009-12-31T23:00:00Z

Title: Migrating azygos vein and vanishing azygos lobe: MDCT findings Author(s) : Villanueva, A. (Alberto); Caceres, J. (José); Ferreira, M. (Mónica); Brocano, J. (Jordi); Pallisa, E. (Esther); Bastarrika, G. (Gorka) Abstract: OBJECTIVE: The purpose of this study was to describe six cases of migrating azygos vein and to explain the etiologic factors that contribute to the migration. Six patients with migrating azygos vein were studied by MDCT before and after migration. Five patients had right pneumothorax. All patients had repeated episodes of cough, vomiting, and a short mesoazygos. CONCLUSION: Pneumothorax, increased intrathoracic pressure, and a short mesoazygos, in combination or alone, are the main factors in azygos vein migration.

2009-12-31T23:00:00Z Levy-Praschker, B.G. (Beltran G.) Bastarrika, G. (Gorka) Dell’Aquila, A.M. (Angelo Maria) Rabago, G. (Gregorio) http://hdl.handle.net/10171/22467 2012-10-03T08:44:14Z 2009-12-31T23:00:00Z

Title: Giant left atrial thrombus 17 years after orthotopic heart transplantation Author(s) : Levy-Praschker, B.G. (Beltran G.); Bastarrika, G. (Gorka); Dell’Aquila, A.M. (Angelo Maria); Rabago, G. (Gregorio) Abstract: We present the case of a 66-year-old woman who underwent orthotopic heart transplantation 17 years earlier for dilated cardiomyopathy. After 7 years allograft coronary vasculopathy developed requiring coronary artery angioplasty. In year 15 postoperatively she experienced congestive heart failure and she became symptomatic requiring diuretics and digoxin treatment. In year 16 postoperatively a routine coronary computed tomography (CT) angiography study revealed a giant thrombus in the left atrium. The patient had had no thromboembolicrelated symptoms. Anticoagulation therapy was introduced and the patient has not presented any thromboembolic-related complication. As the symptoms of cardiac insufficiency worsened we decided to evaluate the patient for re-transplantation.

2009-12-31T23:00:00Z Bastarrika, G. (Gorka) Simon-Yarza, I. (Isabel) Gavira, J.J. (Juan José) http://hdl.handle.net/10171/22411 2012-06-19T10:16:41Z 2010-12-31T23:00:00Z

Title: Incidental computed tomography diagnosis of a rare triad consisting of absence of coronary sinus, persistent left superior vena cava, and scimitar syndrome Author(s) : Bastarrika, G. (Gorka); Simon-Yarza, I. (Isabel); Gavira, J.J. (Juan José) Abstract: We report a case of an unusual congenital triad consisting of absence of coronary sinus, persistent left superior vena cava and scimitar syndrome incidentally found in a CT-scan performed on a female complaining of exertional dyspnea.

2010-12-31T23:00:00Z Ceresa, M. (M.) Bastarrika, G. (G.) Torres, J.P. (Juan P.) de Montuenga, L.M. (Luis M.) Zulueta, J.J. (Javier J.) Ortiz-de-Solorzano, C. (Carlos) Muñoz-Barrutia, A. (Arrate) http://hdl.handle.net/10171/19028 2011-09-19T09:41:19Z 2010-12-31T23:00:00Z

Title: Robust, Standardized Quantification of Pulmonary Emphysema in Low Dose CT Exams Author(s) : Ceresa, M. (M.); Bastarrika, G. (G.); Torres, J.P. (Juan P.) de; Montuenga, L.M. (Luis M.); Zulueta, J.J. (Javier J.); Ortiz-de-Solorzano, C. (Carlos); Muñoz-Barrutia, A. (Arrate) Abstract: RATIONALE AND OBJECTIVES: The aim of this study was to present and evaluate a fully automated system for emphysema quantification on low-dose computed tomographic images. The platform standardizes emphysema measurements against changes in the reconstruction algorithm and slice thickness. MATERIALS AND METHODS: Emphysema was quantified in 149 patients using a fully automatic, in-house developed software (the Robust Automatic On-Line Pulmonary Helper). The accuracy of the system was evaluated against commercial software, and its reproducibility was assessed using pairs of volume-corrected images taken 1 year apart. Furthermore, to standardize quantifications, the effect of changing the reconstruction parameters was modeled using a nonlinear fit, and the inverse of the model function was then applied to the data. The association between quantifications and pulmonary function testing was also evaluated. The accuracy of the in-house software compared to that of commercial software was measured using Spearman's rank correlation coefficient, the mean difference, and the intrasubject variability. Agreement between the methods was studied using Bland-Altman plots. To assess the reproducibility of the method, intraclass correlation coefficients and Bland-Altman plots were used. The statistical significance of the differences between the standardized data and the reference data (soft-tissue reconstruction algorithm B40f; slice thickness, 1 mm) was assessed using a paired two-sample t test. RESULTS: The accuracy of the method, measured as intrasubject variability, was 3.86 mL for pulmonary volume, 0.01% for emphysema index, and 0.39 Hounsfield units for mean lung density. Reproducibility, assessed using the intraclass correlation coefficient, was >0.95 for all measurements. The standardization method applied to compensate for variations in the reconstruction algorithm and slice thickness increased the intraclass correlation coefficients from 0.87 to 0.97 and from 0.99 to 1.00, respectively. The correlation of the standardized measurements with pulmonary function testing parameters was similar to that of the reference (for the emphysema index and the obstructive subgroup: forced expiratory volume in 1 second, -0.647% vs -0.615%; forced expiratory volume in 1 second/forced vital capacity, -0.672% vs -0.654%; and diffusing capacity for carbon monoxide adjusted for hemoglobin concentration, -0.438% vs -0.523%). CONCLUSIONS: The new emphysema quantification method presented in this report is accurate and reproducible and, thanks to its standardization method, robust to changes in the reconstruction parameters.

2010-12-31T23:00:00Z Bastarrika, G. (Gorka) Garcia-Velloso, M. (María José) Lozano, M.D. (María D.) Montes, U. (Usúa) Torre, W. (Wenceslao) Rodriguez-Spiteri, N. (Natalia) Campo, A. (A.) Seijo, L. (Luis) Alcaide, A.B. (Ana Belén) Pueyo, J. (Jesús) Cano, D. (David) Vivas, I. (Isabel) Cosin, O. (Octavio) Dominguez-Echavarri, P. (Pablo) Serra, P. (Patricia) Richter, J.A. (José Ángel) Montuenga, L.M. (Luis M.) Zulueta, J.J. (Javier J.) http://hdl.handle.net/10171/18803 2013-01-08T16:07:39Z 2004-12-31T23:00:00Z

Title: Early lung cancer detection using spiral computed tomography and positron emission tomography Author(s) : Bastarrika, G. (Gorka); Garcia-Velloso, M. (María José); Lozano, M.D. (María D.); Montes, U. (Usúa); Torre, W. (Wenceslao); Rodriguez-Spiteri, N. (Natalia); Campo, A. (A.); Seijo, L. (Luis); Alcaide, A.B. (Ana Belén); Pueyo, J. (Jesús); Cano, D. (David); Vivas, I. (Isabel); Cosin, O. (Octavio); Dominguez-Echavarri, P. (Pablo); Serra, P. (Patricia); Richter, J.A. (José Ángel); Montuenga, L.M. (Luis M.); Zulueta, J.J. (Javier J.) Abstract: RATIONALE: Lung cancer screening using computed tomography (CT) is effective in detecting lung cancer in early stages. Concerns regarding false-positive rates and unnecessary invasive procedures have been raised. OBJECTIVE: To study the efficiency of a lung cancer protocol using spiral CT and F-18-fluorodeoxyglucose positron emission tomography (FDG-PET). METHODS: High-risk individuals underwent screening with annual spiral CTs. Follow-up CTs were done for noncalcified nodules of 5 mm or greater, and FDG-PET was done for nodules 10 mm or larger or smaller (> 7 mm), growing nodules. RESULTS: A total of 911 individuals completed a baseline CT study and 424 had at least one annual follow-up study. Of the former, 14% had noncalcified nodules of 5 mm or larger, and 3.6% had nodules of 10 mm or larger. Eleven non-small cell lung cancers (NSCLC) and one small cell lung cancer (SCLC) were diagnosed in the baseline study (prevalence rate, 1.32%), and two NSCLCs in the annual study (incidence rate, 0.47%). All NSCLCs (92% of prevalence cancers) were diagnosed in stage I (12 stage IA, 1 stage IB). FDG-PET was helpful for the correct diagnosis in 19 of 25 indeterminate nodules. The sensitivity, specificity, positive predictive value, and negative predictive value of FDG-PET for the diagnosis of malignancy were 69, 91, 90, and 71%, respectively. However, the sensitivity and negative predictive value of the screening algorithm, which included a 3-month follow-up CT for nodules with a negative FDG-PET, was 100%. CONCLUSION: A protocol for early lung cancer detection using spiral CT and FDG-PET is useful and may minimize unnecessary invasive procedures for benign lesions.

2004-12-31T23:00:00Z Bastarrika, G. (G.) Gavira, J.J. (Juan José) Abizanda, G. (Gloria) Alonso-Burgos, A. (Alberto) http://hdl.handle.net/10171/18132 2012-07-30T09:03:44Z 2008-12-31T23:00:00Z

Title: Función, volúmenes y masa ventricular izquierda por resonancia magnética en estudios realizados en un modelo animal con secuencias SSFPyFLASH: comparación de los resultados Author(s) : Bastarrika, G. (G.); Gavira, J.J. (Juan José); Abizanda, G. (Gloria); Alonso-Burgos, A. (Alberto) Abstract: Objetivo: Comparar la exactitud de 2 secuencias cine eco degradiente para cuantificar función, volúmenes y masa ventricular izquierda en un modelo animal. Material y métodos: Se estudiaron 10 cerdos miniatura tipo Goettingen(7 machos,3 hembras; peso medio: 49, 8710, 65 kg; rango: 35–65 kg)en un equipo 1, 5 Tesla con secuencias recesión libre en estado estacionario(SSFP)y ecodegradiente convencionales (FLASH) en respiración libre. Se utilizaron imágenes de 8 mm de grosor obtenidas en el eje corto para cuantificar los parámetros del ventrículo izquierdo. Se calcularon la fracción de eyección (FE), volúmenes (volumen telediastólico [VTD], volumen telesistólico [VTS], volumen latido[VL]), masa ventricular y la relación señal ruido(RSR)con cada secuencia. Se estudiaron la correlación y concordancia entre las distintas secuencias para cada variable.

2008-12-31T23:00:00Z Pajares, M.J. (María José) Zudaire, I. (Isabel) Lozano, M.D. (María D.) Agorreta, J. (Jacqueline) Bastarrika, G. (G.) Torre, W. (Wenceslao) Remirez, A. (Ana) http://hdl.handle.net/10171/16768 2012-01-14T18:35:30Z 2006-01-31T23:00:00Z

Title: Molecular profiling of computed tomography screen-detected lung nodules shows multiples malignant features Author(s) : Pajares, M.J. (María José); Zudaire, I. (Isabel); Lozano, M.D. (María D.); Agorreta, J. (Jacqueline); Bastarrika, G. (G.); Torre, W. (Wenceslao); Remirez, A. (Ana) Abstract: Rationale and Purpose: Low-dose spiral computerized axial tomography (spiral CT) is effective for the detection of small early lung cancers. Although published data seem promising, there has been a significant degree of discussion concerning the potential of overdiagnosis in the context of spiral CT–based screening. The objective of the current study was to analyze the phenotypic and genetic alterations in the small pulmonary malignancies resected after detection in the University of Navarra/International Early Lung Cancer Action Project spiral CT screening trial and to determine whether their malignant molecular features are similar to those of resected lung tumors diagnosed conventionally. Experimental Design: We analyzed 17 biomarkers of lung epithelial malignancy in a series of 11 tumors resected at our institution during the last 4 years (1,004 high-risk individuals screened), using immunohistochemistry and fluorescence in situ hybridization (FISH). A parallel series of 11 gender-, stage-, and histology-matched lung cancers diagnosed by other means except screening was used as control. Results: The molecular alterations and the frequency of phenotypic or genetic aberrations were very similar when screen-detected and nonscreen-detected lung cancers were compared. Furthermore, most of the alterations found in the screen-detected cancers from this study were concordant with what has been described previously for stage I-II lung cancer. Conclusions: Small early-stage lung cancers resected after detection in a spiral CT-based screening trial reveal malignant molecular features similar to those found in conventionally diagnosed lung cancers, suggesting that the screen-detected cancers are not overdiagnosed

2006-01-31T23:00:00Z Artaechevarria-Artieda, X. (Xabier) Blanco, D. (D.) Perez-Martin, D. (Daniel) Biurrun, G. (G.) de Montuenga, L.M. (Luis M.) Torres, J.P. (Juan P.) de Zulueta, J.J. (Javier J.) Bastarrika, G. (G.) Muñoz-Barrutia, A. (Arrate) Ortiz-de-Solorzano, C. (Carlos) http://hdl.handle.net/10171/13172 2011-06-08T15:15:25Z 2010-05-23T22:00:00Z

Title: Longitudinal study of a mouse model of chronic pulmonary inflammation using breath hold gated micro-CT. Author(s) : Artaechevarria-Artieda, X. (Xabier); Blanco, D. (D.); Perez-Martin, D. (Daniel); Biurrun, G. (G.) de; Montuenga, L.M. (Luis M.); Torres, J.P. (Juan P.) de; Zulueta, J.J. (Javier J.); Bastarrika, G. (G.); Muñoz-Barrutia, A. (Arrate); Ortiz-de-Solorzano, C. (Carlos) Abstract: Abstract Objectives To evaluate the feasibility of using automatic quantitative analysis of breath hold gated micro-CT images to detect and monitor disease in a mouse model of chronic pulmonary inflammation, and to compare image-based measurements with pulmonary function tests and histomorphometry. Material and methods Forty-nine A/J mice were used, divided into control and inflammation groups. Chronic inflammation was induced by silica aspiration. Fourteen animals were imaged at baseline, and 4, 14, and 34 weeks after silica aspiration, using micro-CT synchronized with ventilator-induced breath holds. Lung input impedance was measured as well using forced oscillation techniques. Five additional animals from each group were killed after micro-CT for comparison with histomorphometry. Results At all time points, micro-CT measurements show statistically significant differences between the two groups, while first differences in functional test parameters appear at 14 weeks. Micro-CT measurements correlate well with histomorphometry and discriminate diseased and healthy groups better than functional tests. Conclusion Longitudinal studies using breath hold gated micro-CT are feasible on the silica-induced model of chronic pulmonary inflammation, and automatic measurements from micro-CT images correlate well with histomorphometry, being more sensitive than functional tests to detect lung damage in this model.

2010-05-23T22:00:00Z