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Dadun > Depósito Académico > Facultad de Ciencias > Departamento de Histología y Anatomía Patológica > DA - Ciencias - HAP - Artículos de revista >

MAPC transplantation confers a more durable benefit than AC133+ cell transplantation
Authors: Aranguren, X.L. (Xabier L.)
Pelacho, B. (Beatriz)
Peñuelas, I. (Iván)
Abizanda, G. (Gloria)
Uriz, M. (Maialen)
Ecay, M. (M.)
Collantes, M. (María)
Araña, M. (M.)
Beerens, M. (M)
Coppiello, G. (G.)
Prieto, J. (Jesús)
Perez-Ilzarbe, M. (Maitane)
Andreu, E.J. (Enrique José)
Luttun, A. (Aernout)
Prosper, F. (Felipe)
Keywords: Mapc
AC133+ cells
Critical limb ischemia
Stem cells
Angiogenesis
Multipotent Adult Progenitor Cells
Ischemia
Micropet
Myoblast
Issue Date: 17-Aug-2010
Publisher: Cognizant Communication Corporation
Publisher version: http://dx.doi.org/10.3727/096368910X516592
ISSN: 0963-6897
Citation: Cell Transplant 2011;20(2):259-269.
Abstract
There is a need for comparative studies to determine which cell types are better candidates to remedy ischemia. Here, we compared human AC133+ cells and Multipotent Adult Progenitor Cells (hMAPC) in a mouse model reminiscent of critical limb ischemia. hMAPC or hAC133+ cell transplantation induced a significant improvement in tissue perfusion (measured by microPET) 15 days post-transplantation compared to controls. This improvement persisted for 30 days in hMAPC-treated but not in hAC133+-injected animals. While transplantation of hAC133+ cells promoted capillary growth, hMAPC transplantation also induced collateral expansion, decreased muscle necrosis/fibrosis and improved muscle regeneration. Incorporation of differentiated hAC133+ or hMAPC progeny into new vessels was limited, however, a paracrine angio/arteriogenic effect was demonstrated in animals treated with hMAPC. Accordingly, hMAPC-, but not hAC133+-conditioned media, stimulated vascular cell proliferation and prevented myoblast, endothelial and smooth muscle cell apoptosis in vitro. Our study suggests that although hAC133+ cell and hMAPC transplantation bothcontribute to vascular regeneration in ischemic limbs, hMAPC exert a more robust effect through trophic mechanisms, which translated into collateral and muscle fiber regeneration. This, in turn, conferred tissue protection and regeneration with longer-term functional improvement.
Permanent link: http://hdl.handle.net/10171/13042
Appears in Collections:DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Servicios de apoyo - Micropet - Artículos de revista
DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista

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