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Dadun > Depósito Académico > Facultad de Ciencias > Departamento de Histología y Anatomía Patológica > DA - Ciencias - HAP - Artículos de revista >

13N-Ammonia PET as a Measurement of Hindlimb Perfusion in a Mouse Model of Peripheral Artery Occlusive Disease
Authors: Peñuelas, I. (Iván)
Aranguren, X.L. (Xabier L.)
Abizanda, G. (Gloria)
Marti-Climent, J.M. (Josep María)
Uriz, M. (Maialen)
Ecay, M. (M.)
Collantes, M. (María)
Quincoces, G. (Gemma)
Richter, J.A. (José Ángel)
Prosper, F. (Felipe)
Keywords: 13N-Ammonia PET
Measurement
Hindlimb Perfusion
Mouse Model
Peripheral Artery Occlusive Disease
Issue Date: 2007
Publisher: SOC NUCLEAR MEDICINE INC
Publisher version: http://dx.doi.org/10.2967/jnumed.106.039180
ISBN: 0161-5505
Citation: Penuelas I, Aranguren XL, Abizanda G, Marti-Climent JM, Uriz M, Ecay M, et al... (13)N-ammonia PET as a measurement of hindlimb perfusion in a mouse model of peripheral artery occlusive disease. J Nucl Med 2007 ; 48(7): 1216-1223
Abstract
Peripheral arterial occlusive disease (PAOD) is a leading cause of mortality and morbidity in the western world. The development of noninvasive methods for assessment and comparison of the efficacy of novel therapies in animal models is of great importance. Methods: Hindlimb ischemia was induced in nude mice by ligation and excision of the left femoral artery (n = 5) or the left iliac artery (n = 10). Assessment of limb perfusion was performed by small-animal PET analysis after intravenous injection of 13N-ammonia between 24 h and 30 d after surgery using the ratio of perfusion between the left limb (ischemic) and the right limb (control). Activity concentration per area unit was calculated in regions of interest placed on 1-mm-thick images for numeric calculations, and the iliac and the femoral models were compared. In addition, histopathologic studies were performed to assess the degree of necrosis (hematoxylin–eosin) and fibrosis (sirius red). Immunohistochemistry analyses for identification of arterioles ({alpha}-smooth muscle actin) and endothelium—capillaries—(Bandeiraea simplicifolia I [BS-I] lectin) were also performed. Results: Perfusion in both hindlimbs of control animals was similar (median of the left-to-right ratio = 0.99). Twenty-four hours after ischemia, perfusion of the ischemic limb (% mean ± SD) was 33.3 ± 10.6 and 22.1 ± 9.9 in the femoral and iliac models, respectively. Spontaneous recovery of perfusion in the hindlimb that underwent surgery was significantly lower in the iliac model at day +15 (73.2 ± 15.5 vs. 51.9 ± 11.3; P < 0.01). Fibrosis increased progressively until day +30, whereas muscle necrosis was maximal at day +7 with a moderate reduction by day +30. In accordance with this positive effect, there was a statistically significant increase in the area covered with smooth muscle-coated vessels (arterioles) at day +30 in comparison with day 7 (P < 0.05). In addition, a correlation between 13N-ammonia uptake and the amount of necrosis (r = –0.73; P = 0.06) and fibrosis (r = –0.67; P = 0.05) at day +30 was found. Conclusion: 13N-Ammonia imaging allows semiquantitative evaluation of hindlimb perfusion in surgical mouse models of acute hindlimb ischemia. Although spontaneous perfusion recovery is observed in both models, the iliac model shows a substantially lower recovery and is hence better suited for assessment of new therapeutic strategies for acute hindlimb
Permanent link: http://hdl.handle.net/10171/16833
Appears in Collections:DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CIMA - Servicios de apoyo - Micropet - Artículos de revista
DA - CIMA - Oncología - Oncología molecular - Artículos de Revista
DA - Ciencias - HAP - Artículos de revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista

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