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Dadun > Depósito Académico > Facultad de Farmacia > Departamento de Ciencias de la Alimentación, Fisiología y Toxicología > DA - Farmacia - CAFT - Artículos de revista >

Lipoic acid inhibits leptin secretion and Sp1 activity in adipocytes
Authors: Prieto-Hontoria, P.L. (Pedro L.)
Perez-Matute, P. (Patricia)
Fernandez-Galilea, M. (M.)
Martinez, J.A. (José Alfredo)
Moreno-Aliaga, M.J. (María Jesús)
Keywords: Nutrición y dietética
Lipoic acid
Obesity
Leptin
Ácido lipoico
Sp1 transcription factor
Obesidad
PI3K
Issue Date: 2011
Publisher: Wiley Blackwell
Publisher version: http://dx.doi.org/10.1002/mnfr.201000534
ISSN: 1613-4125
Citation: Prieto-Hontoria PL, Perez-Matute P, Fernandez-Galilea M, Barber A, Martinez JA, Moreno-Aliaga MJ. Lipoic acid prevents body weight gain induced by a high fat diet in rats: effects on intestinal sugar transport. J Physiol Biochem 2009 Mar;65(1):43-50.
Abstract
Lipoic acid (LA) is an antioxidant with therapeutic potential on several diseases such as diabetes and obesity. Hyperleptinemia and oxidative stress play a major role in the development of obesity-linked diseases. The aim of this study was to examine in vivo and in vitro the effects of LA on leptin production, as well as to elucidate the mechanisms and signalling pathways involved in LA actions. Methods and results: Dietary supplementation with LA decreased both circulating leptin, and adipose tissue leptin mRNA in rats. Treatment of 3T3-L1 adipocytes with LA caused a concentration-dependent inhibition of leptin secretion and gene expression. Moreover, LA stimulated the anaerobic utilization of glucose to lactate, which negatively correlated with leptin secretion. Furthermore, LA enhanced phosphorylation of Sp1 and inhibited Sp1 transcriptional activity in 3T3-L1 adipocytes. Moreover, LA inhibited Akt phosphorylation, a downstream target of phosphatidylinositol 3-kinase (PI3K). Treatment with the PI3K inhibitor LY294002 mimicked LA actions, dramatically inhibiting both leptin secretion and gene expression and stimulating Sp1 phosphorylation. Conclusion: All of these data suggest that the phosphorylation of Sp1 and the accompanying reduced DNA-binding activity are likely to be involved in the inhibition of leptin induced by LA, which could be mediated in part by the abrogation of the PI3K/Akt pathway.
Permanent link: http://hdl.handle.net/10171/16860
Appears in Collections:DA - Farmacia - CAFT - Línea Especial de Nutrición
DA - Farmacia - CAFT - Artículos de revista

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