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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Terapia celular > DA - CIMA - Oncología - Terapia celular - Artículos de Revista >

Identification of recurrent chromosomal breakpoints in multiple myeloma with complex karyotypes by combined G-banding, spectral karyotyping, and fluorescence in situ hybridization analyses
Authors: Saez, B. (Borja)
Martin-Subero, J.I. (José Ignacio)
Largo, C. (Cristina)
Martin, M.C. (María C.)
Odero, M.D. (María D.)
Prosper, F. (Felipe)
Siebert, R. (Reiner)
Calasanz, M.J. (María José)
Cigudosa, J.C. (Juan Cruz)
Keywords: Materias Investigacion::Ciencias de la Salud::Oncología
Issue Date: 2006
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/journal/01654608
ISSN: 0165-4608
Citation: Sáez, B., Martín-Subero, J. J., Largo, C., Martín, M. C. et al. Cancer. Genet. Cytogen. 2006; 169: 143-149
Abstract
The description of novel chromosomal aberrations in multiple myeloma (MM) remains necessary to fully understand the pathogenesis of this heterogeneous disease. Therefore, we have used spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) with locus-specific probes to characterize the chromosomal abnormalities in 11 MM cases in which G-banding revealed a complex karyotype. SKY refined G-banding karyotypes in all cases. Recurrent breakpoints involved bands Xp11, 8q24, 11q13, 12q13, 13q21, and 14q32. In addition, combined SKY and FISH analyses permitted us to identify a subset of patients harboring 22q11.2 rearrangements not involving the IGL locus. This finding suggests the presence of other gene(s) in band 22q11 that might be implicated in MM pathogenesis. Moreover, band 1p13 was identified as a novel partner of immunoglobulin (IG) translocations in MM. Finally, using interphase FISH, we have detected interstitial deletions in 13q14 and 17p13, as well as cryptic translocations affecting IGH, which were neither detected by G-banding nor by SKY. The results of the present study suggest the existence of hitherto unknown nonrandom chromosomal changes that may play a role in the pathogenesis of MM. Our findings underline the importance of the combination of banding, SKY, and FISH analyses to increase the accuracy of karyotype interpretation in plasma cell neoplasias.
Permanent link: http://hdl.handle.net/10171/17418
Appears in Collections:DA - CIMA - Oncología - Genética - Artículos de revista
DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista
DA - Ciencias - Genética - Artículos de revista

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