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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Terapia celular > DA - CIMA - Oncología - Terapia celular - Artículos de Revista >

Multicolor interphase cytogenetics for the study of plasma cell dyscrasias
Authors: Saez, B. (Borja)
Martin-Subero, J.I. (José Ignacio)
Odero, M.D. (María D.)
Prosper, F. (Felipe)
Cigudosa, J.C. (Juan Cruz)
Schoch, R. (Robert)
Calasanz, M.J. (María José)
Siebert, R. (Reiner)
Keywords: Multiple myeloma
In situ hybridization
Fluorescence immunophenotyping
Interphase cytogenetics
Issue Date: 2007
Publisher: Spandidos
Publisher version: http://www.spandidos-publications.com/or/
ISSN: 1021-335X
Citation: Sáez, B., Martín-Subero, J. I., Odero, M. D., Prósper, F., et al. Oncol. Rep. 2007; 18: 1099-1106
Abstract
Specific chromosomal abnormalities such as chromosome 13 deletions and some translocations affecting the immunoglobulin heavy chain (IGH) gene, namely t(4;14)(p16;q32) and t(14;16)(q32;q23) have been associated with an adverse prognosis in multiple myeloma. Conventional cytogenetic techniques fail to detect these aberrations in the majority of cases. Thus, we have developed a novel set of interphase fluorescence in situ hybridization (I-FISH) assays targeting those regions frequently lost on chromosome 13 as well as those oncogenes most recurrently involved in translocations with the IGH locus in multiple myeloma, i.e. IRTA1/2 (1q21), FGFR3/MMSET (4p16), CCND3 (6p21), IRF4 (6p25), CCND1 (11q13), MAF (16q23), and MAFB (20q12). The probes were combined in a multicolor fashion to develop novel multicolor I-FISH (MI-FISH) assays, whose validity and applicability was evaluated in negative controls and in a series of 13 plasma cell neoplasias. Additionally, a combination of the novel MI-FISH assays with staining for the plasma cell-specific antigen VS38c by means of multicolor FICTION (M-FICTION, fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasms) allowed us to selectively analyze the plasma cell compartment, and thereby to increase the assay sensitivity.
Permanent link: http://hdl.handle.net/10171/17697
Appears in Collections:DA - CIMA - Oncología - Genética - Artículos de revista
DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista
DA - Ciencias - Genética - Artículos de revista

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