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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Terapia celular > DA - CIMA - Oncología - Terapia celular - Artículos de Revista >

Epigenetic regulation of PRAME gene in chronic myeloid leukemia
Authors: Roman-Gomez, J. (José)
Jimenez-Velasco, A. (A.)
Aguirre, X. (Xavier)
Castillejo, J.A. (J.A.)
Navarro, G. (Germán)
San-Jose, E. (Edurne)
Garate, L. (L.)
Cordeu, L. (Lucía)
Cervantes, F. (F.)
Prosper, F. (Felipe)
Heiniger, A. (A.)
Torres, A. (Antonio)
Keywords: PRAME
Hypomethylation
CML
Issue Date: 2007
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/journal/01452126
ISSN: 0145-2126
Citation: Román-Gómez, J., Jiménez-Velasco, A., Agirre, X., Castillejo, J. A. et al. Leukemia Research 2007; 31: 1521-1528
Abstract
Tumor associated antigens (TAA) provide attractive targets for cancer-specific immunotherapy. PRAME is a TAA gene up-regulated in advanced phases of chronic myeloid leukemia (CML). To date, molecular mechanisms for the expression of PRAME have never been studied. We found that some Ph’-positive cell lines did not express PRAME. The expression of PRAMEwas restored in these cell lines by treatment with 5 -aza-2 -deoxycytidine, suggesting that the expression of PRAME is mainly suppressed by hypermethylation. Bisulfite sequencing analysis of the CpG sites of the PRAME exon 2 in these cancer cell lines revealed a close relationship between the methylation status of the PRAME gene and its expression. A methylation-specific PCR analysis demonstrated that hypomethylation of PRAME was significantly more frequent in CML blast crisis (70%) than in chronic phase (36%) (P = 0.01) and was correlated with high expression levels of PRAME transcripts (P < 0.0001). These results suggest that hypomethylation of PRAME up-regulates its expression in CML and might play a significant role in the progression of the disease.
Permanent link: http://hdl.handle.net/10171/17701
Appears in Collections:DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista

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