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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Terapia celular > DA - CIMA - Oncología - Terapia celular - Artículos de Revista >

Adipose-derived cardiomyogenic cells: in vitro expansion and functional improvement in a mouse model of myocardial infarction
Authors: Leobon, B. (Bertrand)
Roncalli, J. (Jérôme)
Joffre, C. (Carine)
Mazo, M. (Manuel)
Boisson, M. (M.)
Barreau, C. (C.)
Calise, D. (D.)
Arnaud, E. (E.)
Andre, M. (M.)
Pucéat, M. (Michel)
Penicaud, L. (Luc)
Prosper, F. (Felipe)
Planat-Benard, V. (Valérie)
Casteilla, L. (L.)
Keywords: Adipose-derived stem cells
Cardiomyocyte
Acute myocardial infarction
Ischaemia
Cell therapy
Issue Date: 2009
Publisher: Oxford University Press
Publisher version: http://dx.doi.org/10.1093/cvr/cvp167
ISSN: 0008-6363
Citation: Léobon, B., Roncalli, J., Joffre, C., Mazo, M. et al. Adipose-derived cardiomyogenic cells: in vitro expansion and functional improvement in a mouse model of myocardial infarction. Cardiovasc. Res. (2009); 83 (4): 757-767
Abstract
Aims Cells derived from the stroma vascular fraction (SVF) of mouse adipose tissue can spontaneously give rise to rare, functional, cardiac-like cells in vitro. This study aimed to improve the production of adipose-derived cardiomyogenic cells (AD-CMG), to characterize them and to assess their cardiac fate and functional outcomes after their administration in a mouse model of acute myocardial infarction. Methods and results The culture process optimized to improve in vitro cardiac specification consisted of a primary culture of murine SVF cells in semi-solid methylcellulose medium, a selection of AD-CMG cell clusters, and a secondary culture and expansion in BHK21 medium. AD-CMG cells were CD29+, CD31−, CD34−, CD44+, CD45−, CD81+, CD90−, CD117−, and Flk-1− and expressed several cardiac contractile proteins. After 1, 2, and 4 weeks of their injection in mice having acute myocardial infarction, a strong presence of green fluorescent protein-positive cells was identified by immunohistochemistry as well as quantitative polymerase chain reaction. Echocardiography showed a significant reduction of remodelling and stability of left ventricle ejection fraction in the AD-CMG cell-treated group vs. controls. Vascular density analysis revealed that AD-CMG administration was also associated with stimulation of angiogenesis in peri-infarct areas. Conclusion Cardiomyogenic cells can be selected and expanded in large amounts from mouse adipose tissue. They can survive and differentiate in an acute myocardial infarction model, avoiding remodelling and impairment of cardiac function, and can promote neo-vascularization in the ischaemic heart.
Permanent link: http://hdl.handle.net/10171/18022
Appears in Collections:DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista

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