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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Terapia celular > DA - CIMA - Oncología - Terapia celular - Artículos de Revista >

Multipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cells
Authors: Rice, C.M. (Claire .M)
Clavel, C. (C.)
Mazo, M. (Manuel)
Prosper, F. (Felipe)
Scolding, N.J. (Neil J.)
Keywords: Multipotent adult progenitor cells,
B104
Cell culture
Cell growth
Proliferation
Materias Investigacion::Ciencias de la Salud::Oncología
Issue Date: 2010
Publisher: Wiley-Blackwell
Publisher version: http://dx.doi.org/10.1111/j.1365-2141.2009.07986.x
ISSN: 0007-1048
Citation: Rice, C. M., Clavel, C., Mazo, M., Prosper, F. et al.Multipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cells. Br. J. Haematol. 2010; 148 (3): 441–444
Abstract
This report describes the isolation of rodent multipotent adult progenitor cells (MAPCs) and proliferation of these cells in both standard medium and medium without exogenous serum or growth factors conditioned by the rat cell line B104. MAPCs have exacting requirements for their proliferation in vitro but once established proliferate rapidly at low seeding density, requiring almost daily passage and media exchange. Previously published methods for growth of MAPCs in vitro all used media supplemented with serum and growth factors, which adds considerable expense.
Permanent link: http://hdl.handle.net/10171/18156
Appears in Collections:DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista

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