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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Terapia celular > DA - CIMA - Oncología - Terapia celular - Artículos de Revista >

Sustained release of VEGF through PLGA microparticles improves vasculogenesis and tissue remodeling in an acute myocardial ischemia–reperfusion model
Authors: Formiga, F.R. (Fabio R.)
Pelacho, B. (Beatriz)
Garbayo, E. (Elisa)
Abizanda, G. (Gloria)
Gavira, J.J. (Juan José)
Simon-Yarza, T. (Teresa)
Mazo, M. (Manuel)
Tamayo, E. (Esther)
Jauquicoa, C. (Carlos)
Ortiz-de-Solorzano, C. (Carlos)
Prosper, F. (Felipe)
Blanco-Prieto, M.J. (María José)
Keywords: Angiogenesis
PLGA microparticles
Controlled release
Myocardial infarction
Issue Date: 2010
Publisher: Elsevier
Publisher version:
ISSN: 0168-3659
Citation: Formiga FR, Pelacho B, Garbayo E, Abizanda G, et al. Sustained release of VEGF through PLGA microparticles improves vasculogenesis and tissue remodeling in an acute myocardial ischemia–reperfusion model. J Control Release 2010;147(1):30–37
The use of pro-angiogenic growth factors in ischemia models has been associated with limited success in the clinical setting, in part owing to the short lived effect of the injected cytokine. The use of a microparticle system could allow localized and sustained cytokine release and consequently a prolonged biological effect with induction of tissue revascularization. To assess the potential of VEGF165 administered as continuous release in ischemic disease, we compared the effect of delivery of poly(lactic–co-glycolic acid) (PLGA) microparticles (MP) loaded with VEGF165 with free-VEGF or control empty microparticles in a rat model of ischemia–reperfusion. VEGF165 loaded microparticles could be detected in the myocardium of the infarcted animals for more than a month after transplant and provided sustained delivery of active protein in vitro and in vivo. One month after treatment, an increase in angiogenesis (small caliber caveolin-1 positive vessels) and arteriogenesis (α-SMA-positive vessels) was observed in animals treated with VEGF microparticles (pb0.05), but not in the empty microparticles or free-VEGF groups. Correlating with this data, a positive remodeling of the heart was also detected in the VEGF-microparticle group with a significantly greater LV wall thickness (pb0.01). In conclusion, PLGA microparticle is a feasible and promising cytokine delivery system for treatment of myocardial ischemia. This strategy could be scaled up and explored in pre-clinical and clinical studies.
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Appears in Collections:DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Imagen del cáncer - Artículos de revista
DA - CIMA - Unidad de Imagen - Artículos de revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - Farmacia - Tecnología Farmacéutica - Artículos de revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista

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