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|Title: ||Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular cardiomyoplasty|
|Author(s) : ||Chachques, J. (J.)|
Herreros, J. (Jesús)
Trainini, J. (Jorge)
Juffe, A. (A.)
Rendal, E. (Esther)
Prosper, F. (Felipe)
Genovese, J. (J.)
|Issue Date: ||2004|
|Citation: ||Chachques, J. C., Herreros, J., Trainini, J., Juffe, A., Rendal, E. et al. Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular cardiomyoplasty. International Journal of Cardiology 2004; 95 (suppl. 1): s29-s33|
|Keywords: ||Heart failure|
|Abstract: ||Background: Current clinical experience with cellular cardiomyoplasty (using serum bovine-cultivated myoblasts) has demonstrated
significant malignant ventricular arrhythmias and sudden deaths in patients. In some ongoing clinical trials the implantation of cardioverterdefibrillator
is mandatory. We have hypothesized that contact of human cells with fetal bovine serum results after 3-week fixation of animal
proteins on the cell surface, representing an antigenic substrate for immunological and inflammatory adverse events.
Methods and Results: Autologous myoblasts were transplanted into infarcted LV in 20 patients (90% males, mean age 62±8 years).
Cells were cultivated in a complete human medium during 3 weeks, using the patients' own serum obtained from a blood sample or from
plasmapheresis. Injections were performed during CABG (2.1 grafts/pt).
All patients had an uneventful recovery. At a mean follow-up of 14±5 months without mortality, no malignant cardiac arrhythmias are
reported. LV ejection fraction improved from 28±3% to 52:k4.7% (p = 0.03), and regional wall motion score index (WMSI) from 3.1 to
1.4 (p = 0.04) in the cell-treated segments. Myocardial viability tests showed areas of regeneration. Patients moved from mean NYHA
class 2.5 to class 1.2.
Conclusions: A total autologous cell culture procedure was used in cellular cardiomyoplasty reducing the risk of arrhythmia.
Human-autologous-serum cell expansion avoids the risk of prion, viral or zoonoses contamination. Since patients treated with noncultivated
bone marrow cells are free of arrhythmia, the bovine-culture medium seems to be responsible for this complication. Cellular
cardiomyoplasty may be efficient to avoid progression of ventricular remodeling and subsequent heart failure in ischemic heart disease.|
|Publisher version (URL): ||http://dx.doi.org/10.1016/S0167-5273(04)90009-5|
|Appears in Collections:||DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista|
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista
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