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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Genética > DA - CIMA - Oncología - Genética - Artículos de revista >

De novo erythroleukemia chromosome features include multiple rearrangements, with special involvement of chromosomes 11 and 19
Authors: Cigudosa, J.C. (Juan Cruz)
Odero, M.D. (María D.)
Calasanz, M.J. (María José)
Sole, F. (Francesc)
Salido, M. (Marta)
Arranz, E. (Eva)
Martinez-Ramirez, A. (Angel)
Urioste, M. (Miguel)
Alvarez, S. (Sara)
Cervera, J.V. (Jose V.)
MacGrogan, D. (Donald)
Sanz, M.A. (Miguel A.)
Nimer, S.D. (Stephen D.)
Benítez, J. (José)
Keywords: Chromosome Aberrations
Chromosomes, Human, Pair 11/genetics
Chromosomes, Human, Pair 19/genetics
Gene Rearrangement/genetics
Leukemia, Erythroblastic, Acute/genetic
Issue Date: 2003
Publisher: Wiley-Blackwell
Publisher version: http://dx.doi.org/10.1002/gcc.10180
ISSN: 1098-2264
Citation: Cigudosa JC, Odero MD, Calasanz MJ, Sole F, Salido M, Arranz E, et al. De novo erythroleukemia chromosome features include multiple rearrangements, with special involvement of chromosomes 11 and 19. Genes Chromosomes Cancer 2003 Apr;36(4):406-412.
Abstract
Erythroid leukemia (ERL or AML-M6) is an uncommon subtype of acute myeloid leukemia, the clinical, morphological, and genetic behavior of which needs further characterization. We analyzed a homogeneous group of 23 de novo AML-M6 patients whose bone marrow cells showed complex karyotypes. We also analyzed eight leukemia cell lines with erythroid phenotype, performing detailed molecular cytogenetic analyses, including spectral karyotyping (SKY) in all samples. The main features are: (1) A majority of patients (56%) had hypodiploidy. Loss of genetic material was the most common genetic change, especially monosomies of chromosome 7 or 18, and deletions of chromosome arm 5q. Taken together, 87% of the cases displayed aberrations involving chromosome 5 or 8. (2) We describe a novel, cryptic, and recurrent translocation, t(11;19)(p11.2;q13.1). Another translocation, t(12;21)(p11.2;q11.2), was found to be recurrent in a patient with ERL and in the K562 cell line. (3) MLL gene rearrangements were detected in 20% of cases (three translocations and three amplifications) and, overall, we defined 52 rearrangements (excluding deletions) with a mean of 2.3 translocations per patient. (4) Of the structural aberrations, 21% involved chromosomes 11 and 19. Most of the rearrangements were unbalanced; only 13 reciprocal translocations were observed. The general picture of chromosomal aberrations in cell lines did not reflect what occurred in patient samples. However, both primary samples and cell lines shared three common breakpoints at 19q13.1, 20q11.2, and 21q11.2. This is the first molecular cytogenetic description of the karyotype abnormalities present in patients with ERL. It should assist in the identification of genes involved in erythroleukemogenesis.
Permanent link: http://hdl.handle.net/10171/19598
Appears in Collections:DA - CIMA - Oncología - Genética - Artículos de revista
DA - Ciencias - Genética - Artículos de revista

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