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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neuroanatomía ganglios basales > DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista >

Multiple neuroanatomical tracing in primates
Authors: Lanciego, J. (J.L.)
Luquin, M.R. (María Rosario)
Guillen, J. (Javier)
Gimenez-Amaya, J.M. (José Manuel)
Keywords: Axonal tracing
Fluoro-Gold
Cholera toxin
BDA
HRP
V-VIP
Peroxidase
Neuroanatomy
Issue Date: 1998
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S1385299X98000075
ISSN: 1385-299X
Citation: Lanciego JL, Luquin MR, Guillen J, Gimenez-Amaya JM. Multiple neuroanatomical tracing in primates. Brain Res Brain Res Protoc 1998 Jun;2(4):323-332.
Abstract
The present report deals with a multiple tract-tracing procedure in non-human primates enabling the simultaneous visualization of retrogradely transported Fluoro-Gold (FG) and cholera toxin B subunit (CTB) in combination with anterogradely transported biotinylated dextran amine (BDA). Two issues have played key roles on the achievement of this reliable procedure: first, the recent development of a commercial antiserum against FG that allows us to convert the original fluorescent signal of this dye in a permanent precipitate via standard peroxidase-anti-peroxidase methods; second, the introduction of the novel peroxidase substrate Vector(R) VIP (V-VIP), resulting in a purple precipitate. The combination of these neuroanatomical tracers in one and the same histological section opens a possibility for the permanent visualization of the convergence of inputs from a particular brain area onto identified, two different subsets of projection cells of another area. Furthermore, this combination of three tracers emerges as a powerful technical tool for obtaining broad amounts of complementary data regarding the monkey brain connectivity, thus significantly reducing the number of animals needed to complete a particular study.
Permanent link: http://hdl.handle.net/10171/19836
Appears in Collections:DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista

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