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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neuroanatomía ganglios basales > DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista >

Multiple axonal tracing: simultaneous detection of three tracers in the same section
Authors: Lanciego, J.L. (José Luis)
Wouterlood, F.G. (Floris G.)
Erro, E. (E.)
Gimenez-Amaya, J.M. (José Manuel)
Keywords: Tract-tracing
PHA-L
BDA
Fluoro-Gold
V-VIP
Peroxidase
Issue Date: 1998
Publisher: Springer Verlag
Publisher version: http://www.springerlink.com/content/q5l4acg4bcquqt4l/
ISSN: 1432-119X
Citation: Lanciego JL, Wouterlood FG, Erro E, Gimenez-Amaya JM. Multiple axonal tracing: simultaneous detection of three tracers in the same section. Histochem Cell Biol 1998 Nov;110(5):509-515.
Abstract
Multiple neuroanatomical tract-tracing methods are important tools for elucidating the connectivity between different populations of neurons. Evaluation of the question as to whether two specific fiber inputs converge on a particular, identified population of projection neurons requires the application of a triple-staining procedure that allows the unequivocal detection of three markers in a single section. The present report deals with a combination of tracing methods using anterogradely transported Phaseolus vulgaris leucoagglutinin and biotinylated dextran amine in conjunction with retrogradely transported Fluoro-Gold. These tracers were simultaneously detected according to a three-color paradigm, which includes the use of three different peroxidase substrates (nickel-enhanced diaminobenzidine, diaminobenzidine, and Vector VIP), thus resulting in three distinct precipitates: black, brown, and purple. We illustrate this method by showing convergence of projections arising from neurons located in two separate basal ganglia-related nuclei onto identified thalamostriatal projection neurons.
Permanent link: http://hdl.handle.net/10171/19865
Appears in Collections:DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista

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