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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neuroanatomía ganglios basales > DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista >

Notes on the combined use of V-VIP and DAB peroxidase substrates for the detection of colocalising antigens
Authors: Lanciego, J.L. (José Luis)
Gimenez-Amaya, J.M. (José Manuel)
Keywords: Cholera Toxin/analysis
Neurons/chemistry
Tyrosine 3-Monooxygenase/analysis
Issue Date: 1999
Publisher: Springer Verlag
Publisher version: http://www.springerlink.com/content/wueur9j1rvgv3ycj/
ISSN: 1432-119X
Citation: Lanciego JL, Gimenez-Amaya JM. Notes on the combined use of V-VIP and DAB peroxidase substrates for the detection of colocalising antigens. Histochem Cell Biol 1999 Apr;111(4):305-311.
Abstract
The purpose of the present report was to investigate to what extent the new peroxidase substrate Vector VIP (V-VIP) can be used in combination with DAB chromogen for the unequivocal and permanent detection of colocalising antigens within a single neurone, according to a two-colour paradigm. With this aim, retrograde tract-tracing with cholera toxin B subunit (CTB) or fluoro-gold (FG) was performed to disclose individual, identified subpopulations of neurones in the primate substantia nigra projecting to the caudate nucleus or to the putamen, respectively. Each tracer was detected by means of a PAP procedure and finally stained brown using DAB as a chromogen. Subsequently, both series of sections were processed for the immunocytochemical detection of tyrosine hydroxylase (TH). TH-immunoreactive neurones were stained purple with the peroxidase substrate V-VIP. As a result of the present procedure, several cell bodies of projection neurones, stained brown, can easily be identified within the primate substantia nigra. Some of these neurones additionally displayed purple TH immunoreaction product located in the neuronal dendrites. By contrast, CTB- or FG-unlabelled neurones only show the typical purple precipitate that belongs to V-VIP substrate, both in the cell body as well as in the dendrites.
Permanent link: http://hdl.handle.net/10171/19867
Appears in Collections:DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista

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