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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neuroanatomía ganglios basales > DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista >

Purification of bioactive glycosylated recombinant glial cell line-derived neurotrophic factor
Authors: Garbayo, E. (Elisa)
Ansorena, E. (Eduardo)
Lanciego, J.L. (José Luis)
Aymerich, M.S. (María S.)
Blanco-Prieto, M.J. (María José)
Keywords: GDNF
Parkinson's disease
Protein purification
Issue Date: 2007
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S0378517307002992
ISSN: 1873-3476
Citation: Garbayo E, Ansorena E, Lanciego JL, Aymerich MS, Blanco-Prieto MJ. Purification of bioactive glycosylated recombinant glial cell line-derived neurotrophic factor. Int J Pharm 2007 Nov 1;344(1-2):9-15.
Abstract
Glial cell line-derived neurotrophic factor (GDNF) neuroprotective effect on dopaminergic neurons has been described in vitro and in vivo, turning up as a promising drug for the treatment of Parkinson's disease. Unglycosylated bacteria-obtained GDNF has already been successfully delivered for a long period of time through an infusion pump directly to the putamen of Parkinsonian patients. Nevertheless, improved distribution and safety issues need to be solved and alternative strategies to long-term delivery seem necessary. The use of glycosylated GDNF could eliminate some safety concerns regarding the presence of antibodies against exogenous unglycosylated GDNF used for the treatment. Therefore, we have chosen a mammalian expression system as a source of glycosylated GDNF. In the present work, we describe the purification of recombinant rat GDNF from the culture media of baby hamster kidney (BHK) cells through several purification steps. Highly pure N-glycosylated recombinant GDNF has been obtained similar to the endogenous protein. Furthermore, the purified protein is biologically active when tested its ability to induce PC12 neurite outgrowth.
Permanent link: http://hdl.handle.net/10171/20046
Appears in Collections:DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista

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