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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neuroanatomía ganglios basales > DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista >

Complex brain circuits studied via simultaneous and permanent detection of three transported neuroanatomical tracers in the same histological section.
Authors: Lanciego, J.L. (José Luis)
Wouterlood, F.G. (Floris G.)
Erro, E. (E.)
Arribas, J. (J.)
Gonzalo, N. (Nancy)
Urra, X. (X.)
Cervantes, S. (S.)
Gimenez-Amaya, J.M. (José Manuel)
Keywords: Chromogen
Immunocytochemistry
Dextran amine
Cholera toxin
Fluoro-Gold
Axonal tracing
Neuroanatomy
Issue Date: 2000
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S0165027000003022
ISSN: 1872-678X
Citation: Lanciego JL, Wouterlood FG, Erro E, Arribas J, Gonzalo N, Urra X, et al. Complex brain circuits studied via simultaneous and permanent detection of three transported neuroanatomical tracers in the same histological section. J Neurosci Methods 2000 Nov 15;103(1):127-135.
Abstract
Experimental neuroanatomical tracing methods lie at the basis of the study of the nervous system. When the scientific question is relatively straightforward, it may be sufficient to derive satisfactory answers from experiments in which a single neuroanatomical tracing method is applied. In various scientific paradigms however, for instance when the degree of convergence of two different projections on a particular cortical area or subcortical nucleus is the subject of study, the application of single tracing methods can be either insufficient or uneconomical to solve the questions asked. In cases where chains of projections are the subjects of study, the simultaneous application of two tracing methods or even more may be compulsory. The present contribution focuses on combinations of several neuroanatomical tract-tracing strategies, enabling in the end the simultaneous, unambiguous and permanent detection of three transported markers according to a three-color paradigm. A number of combinations of three tracers or of two tracers plus the immunocytochemical detection of a neuroactive substance can be conceived; we describe several of these combinations implemented by us using the present multitracer protocol.
Permanent link: http://hdl.handle.net/10171/20065
Appears in Collections:DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista

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