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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neuroanatomía ganglios basales > DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista >

Neuroanatomical tracing combined with in situ hybridization: analysis of gene expression patterns within brain circuits of interest
Authors: Conte-Perales, L. (L.)
Barroso-Chinea, P. (P.)
Rico, A.J. (Alberto J.)
Gomez-Bautista, V. (V.)
Lopez, I.P. (Iciar P.)
Roda, E. (Elvira)
Wouterlood, F.G. (Floris G.)
Lanciego, J.L. (José Luis)
Keywords: Retrograde tracing
Fluoro-Gold
Cholera toxin
vGlut
GAD
Basal ganglia
Issue Date: 2010
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S0165027009006190
ISSN: 1872-678X
Citation: Conte-Perales L, Barroso-Chinea P, Rico AJ, Gomez-Bautista V, Lopez IP, Roda E, et al. Neuroanatomical tracing combined with in situ hybridization: analysis of gene expression patterns within brain circuits of interest. J Neurosci Methods 2010 Dec 15;194(1):28-33.
Abstract
Most of our current understanding of brain circuits is based on hodological studies carried out using neuroanatomical tract-tracing. Our aim is to advance one step further by visualizing the functional correlate in a given circuit. In this regard, we believe it is feasible to combine retrograde tracing with fluorescence, non-radioactive in situ hybridization (ISH) protocols. The subsequent detection at the single-cell level of the expression of a given mRNA within retrograde-labeled neurons provides information regarding cellular function. This may be of particular interest when trying to elucidate the performance of brain circuits of interest in animal models of brain diseases. Several combinations of retrograde tracing with either single- and double-ISH are presented here, together with some criteria that influence the selection of the tracer to be used in conjunction with the strong demands of the ISH.
Permanent link: http://hdl.handle.net/10171/20096
Appears in Collections:DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista

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