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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neuroanatomía ganglios basales > DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista >

A sequential protocol combining dual neuroanatomical tract-tracing with the visualization of local circuit neurons within the striatum
Authors: Gonzalo, N. (Nancy)
Moreno, A. (Ainhoa)
Erdozain, M. (M. A.)
Garcia, P. (Pedro)
Vazquez, A. (Alfonso)
Castle, M. (M.)
Lanciego, J. (J.L.)
Keywords: Dextran amine
Fluoro-Gold
Interneuron
Issue Date: 2001
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S016502700100440X
ISSN: 1872-678X
Citation: Gonzalo N, Moreno A, Erdozain MA, Garcia P, Vazquez A, Castle M, et al. A sequential protocol combining dual neuroanatomical tract-tracing with the visualization of local circuit neurons within the striatum. J Neurosci Methods 2001 Oct 15;111(1):59-66.
Abstract
We describe here an experimental approach designed to aid in the identification of complex brain circuits within the rat corpus striatum. Our aim was to characterize in a single section (i) striatal thalamic afferents, (ii) striatopallidal projection neurons and (iii) striatal local circuit interneurons. To this end, we have combined anterograde tracing using biotinylated dextran amine and retrograde neuroanatomical tracing with Fluoro-Gold. This dual tracing protocol was further implemented with the visualization of different subpopulations of striatal interneurons. The subsequent use of three different peroxidase substrates enabled us to unequivocally detect structures that were labeled within a three-color paradigm.
Permanent link: http://hdl.handle.net/10171/20099
Appears in Collections:DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista

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