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Simultaneous immunostaining method for localization of bromodeoxyuridine and calcitonin gene-related peptide
Authors: Montuenga, L.M. (Luis M.)
Springall, D.R. (David R.)
Gaer, J. (J.)
McBride, J.T. (J.T.)
Polak, J.M. (Julia M.)
Keywords: 5'- bromodeoxyuridine
Calcitonin gene-related peptide
Lung
Endocrine cells
Immunohistochemistry
Double immunostaining method
Issue Date: 1992
Publisher: Histochemical Society
Publisher version: http://jhc.sagepub.com/content/40/8/1121
ISSN: 1551-5044
Citation: Montuenga LM, Springall DR, Gaer J, McBride JT, Polak JM. Simultaneous immunostaining method for localization of bromodeoxyuridine and calcitonin gene-related peptide. J Histochem Cytochem 1992 Aug;40(8):1121-1128.
Abstract
A new simultaneous double immunostaining method has been optimized to localize the DNA synthesis marker bromodeoxyuridine (BrdU) and calcitonin gene-related peptide (CGRP) in endocrine cells of Bouin's-fixed, paraffin-embedded rat lung. Nuclease pre-treatment before immunostaining is compatible with optimal tissue morphology and CGRP antigenicity preservation. Nickel-enhanced development of avidin-biotin-peroxidase staining is used to show CGRP immunoreactivity in black and alkaline phosphatase-anti-alkaline phosphatase is applied to demonstrate incorporated BrdU in red. The present methodology could be useful for studies requiring detection of incorporated BrdU in cells producing regulatory peptides or other labile antigens.
Permanent link: http://hdl.handle.net/10171/20111
Appears in Collections:DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Oncología - Biomarcadores - Artículos de Revista

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