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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Biomarcadores > DA - CIMA - Oncología - Biomarcadores - Artículos de Revista >

Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate
Authors: Jimenez, N. (Nuria)
Calvo, A. (Alfonso)
Rosell, D. (David)
Cuttitta, F. (Frank)
Montuenga, L.M. (Luis M.)
Martinez, A. (Alfredo)
Keywords: Adrenomedullin
Proadrenomedullin N-terminal 20 peptide
Adrenomedullin receptor
Prostate
In situ hybridization
RT-PCR
Western blot
Issue Date: 1999
Publisher: Histochemical Society
Publisher version: http://jhc.sagepub.com/content/47/9/1167
ISSN: 1551-5044
Citation: Jimenez N, Calvo A, Martinez A, Rosell D, Cuttitta F, Montuenga LM. Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate. J Histochem Cytochem 1999 Sep;47(9):1167-1178.
Abstract
Adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two recently discovered hypotensive peptides translated from the same message transcript (preproAM mRNA). In this article we report the presence of AM, PAMP, and their mRNA in human and rat prostate and of AM receptor mRNA in rat prostate. PreproAM mRNA was found in the epithelium of normal human and rat prostate glands by in situ hybridization. In humans, it was mainly expressed in the basal cells. In rat, its expression was higher in the ducts than in the acini of all the prostate lobes. Immunocytochemistry identified a similar distribution pattern for AM compared with its mRNA but showed different locations for AM and PAMP immunoreactivity. The former was widespread in the epithelia, whereas the latter was almost exclusively found in neuroendocrine cells. In rat, Western blot analysis confirmed the presence of high levels of AM peptide in the ventral lobe and of its precursor in the ventral and dorsolateral lobes. Immunoreactivity for serotonin, chromogranin A, PAMP, and AM defined four subpopulations of prostate neuroendocrine-like cells in rat, a cell type that has not been previously described.
Permanent link: http://hdl.handle.net/10171/20168
Appears in Collections:DA - CIMA - Oncología - Nuevas dianas terapéuticas - Artículos de revista
DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Oncología - Biomarcadores - Artículos de Revista

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