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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neurofarmacología y conducta > DA - CIMA - Neurociencias - Neurofarmacología y conducta - Artículos de Revista >

Lack of localization of 5-HT6 receptors on cholinergic neurons: implication of multiple neurotransmitter systems in 5-HT6 receptor-mediated acetylcholine release
Authors: Marcos, B. (Beatriz)
Gil-Bea, F.J. (Francisco J.)
Hirst, W.D. (Warren D.)
Garcia-Alloza, M. (Mónica)
Ramirez, M.J. (María Javier)
Keywords: Cholinergic lesion
Dopaminergic system
Glutamatergic system
In vitro release
Rat
Issue Date: 2006
Publisher: Blackwell Publishing
Publisher version: http://onlinelibrary.wiley.com/doi/10.1111/j.1460-9568.2006.05003.x/abstract
ISSN: 1460-9568
Citation: Marcos B, Gil-Bea FJ, Hirst WD, Garcia-Alloza M, Ramirez MJ. Lack of localization of 5-HT6 receptors on cholinergic neurons: implication of multiple neurotransmitter systems in 5-HT6 receptor-mediated acetylcholine release. Eur J Neurosci 2006 Sep;24(5):1299-1306.
Abstract
The involvement of the cholinergic system in learning and memory together with the cognitive enhancing properties of 5-HT6 receptor antagonists led us to study the relationship between 5-HT6 receptors and cholinergic neurotransmission. A selective cholinergic lesion, induced by injection of the immunotoxin 192-IgG-Saporin into the nucleus basalis magnocellularis, failed to alter the density of 5-HT6 receptor mRNA or protein expression in the deafferentated frontal cortex, suggesting that 5-HT6 receptors are not located on cholinergic neurons. The 5-HT6 receptor antagonist SB-357134 (0.001-1 microM) induced a concentration-dependant K+-evoked [3H]acetylcholine (ACh) release in vitro in rat cortical and striatal slices, which was blocked by tetrodotoxin. SB-357134, up to 1 microM, stimulated glutamate release in cortical and striatal slices. In the cortex, riluzole (1 microM) blocked the SB-357134-induced K+-stimulated [3H]ACh release, and simultaneous administration of MK-801 (1 microM) and SB-357134 (0.05 microM) elicited an increase in K+-evoked ACh release. In the striatum, SB-357134, 1 microM, decreased dopamine release, and the increase in K+-evoked [3H]ACh release induced by 5-HT6 receptor blockade was reversed by the D1 receptor antagonist, SCH23390 (1 microM). In both the frontal cortex and striatum, bicuculline, 1 microM, showed no effect on SB-357134-evoked [3H]ACh. These results are discussed in terms of neurochemical mechanisms involved in 5-HT6 receptor functions.
Permanent link: http://hdl.handle.net/10171/20198
Appears in Collections:DA - Farmacia - Farmacología - Artículos de revista
DA - CIMA - Neurociencias - Neurofarmacología y conducta - Artículos de Revista

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