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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Genética molecular > DA - CIMA - Terapia génica y Hepatología - Genética molecular - Artículos de Revista >

Shared apical sorting of anion exchanger isoforms AE2a, AE2b1, and AE2b2 in primary hepatocytes
Authors: Aranda, V. (Victoria)
Martinez, I. (Íñigo)
Melero, S. (Saida)
Lecanda, J. (Jon)
Banales, J.M. (Jesús M.)
Prieto, J. (Jesús)
Medina, J.F. (Juan Francisco)
Keywords: Alternate promoters
Biliary bicarbonate secretion
HEK293 cells
Kidney epithelial MDCK cells
Sandwiched repolarized hepatocytes
Issue Date: 2004
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S0006291X04010575
ISSN: 1090-2104
Citation: Aranda V, Martinez I, Melero S, Lecanda J, Banales JM, Prieto J, et al. Shared apical sorting of anion exchanger isoforms AE2a, AE2b1, and AE2b2 in primary hepatocytes. Biochem Biophys Res Commun 2004 Jul 2;319(3):1040-1046.
Abstract
AE2 (SLC4A2) is the member of the Na(+)-independent anion exchanger (AE) family putatively involved in the secretion of bicarbonate to bile. In humans, three variants of AE2 mRNA have been described: the full-length transcript AE2a (expressed from the upstream promoter in most tissues), and alternative transcripts AE2b(1) and AE2b(2) (driven from alternate promoter sequences in a tissue-restricted manner, mainly in liver and kidney). These transcripts would result in AE protein isoforms with short N-terminal differences. To ascertain their translation, functionality, and membrane sorting, we constructed expression vectors encoding each AE2 isoform fused to GFP at the C-terminus. Transfected HEK293 cells showed expression of functional GFP-tagged AE2 proteins, all three isoforms displaying comparable AE activities. Primary rat hepatocytes transfected with expression vectors and repolarized in a collagen-sandwich configuration showed a microtubule-dependent apical sorting of each AE2 isoform. This shared apical sorting is liver-cell specific, as sorting of AE2 isoforms was basolateral in control experiments on polarized kidney MDCK cells. Hepatocytic apical targeting of AE2 isoforms suggests that they all may participate in the canalicular secretion of bicarbonate to bile.
Permanent link: http://hdl.handle.net/10171/20245
Appears in Collections:DA - CIMA - Terapia génica y Hepatología - Genética molecular - Artículos de Revista

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