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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología hepatitis virales > DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista >

Induction of potent and long-lasting CD4 and CD8 T-cell responses against hepatitis C virus by immunization with viral antigens plus poly(I:C) and anti-CD40
Authors: Zabaleta, A. (Aintzane)
Arribillaga, L. (Laura)
Llopiz, D. (Diana)
Dotor, J. (Javier)
Lasarte, J.J. (Juan José)
Prieto, J. (Jesús)
Borras-Cuesta, F. (Francisco)
Esteban, J.I. (Juan I.)
Quer, J. (Josep)
Vayreda, F. (Francesc)
Sarobe, P. (Pablo)
Keywords: Hepatitis C
Vaccination
Adjuvant
Dendritic cell
T-cell
Issue Date: 2007
Publisher: Elsevier masson
Publisher version: http://dx.doi.org/10.1016/j.antiviral.2007.01.001
ISSN: 0166-3542
Citation: Zabaleta A, Arribillaga L, Llopiz D, Dotor J, Lasarte JJ, Prieto J, et al. Induction of potent and long-lasting CD4 and CD8 T-cell responses against hepatitis C virus by immunization with viral antigens plus poly(I:C) and anti-CD40. Antiviral Res 2007 Apr;74(1):25-35.
Abstract
Development of vaccination strategies against hepatitis C virus (HCV) is of paramount importance. With this aim, we tested the ability of dendritic cell-activating reagents polyinosinic-polycytidylic acid (poly(I:C)) and anti-CD40, as adjuvants to induce T-cell responses against HCV. Immunization of mice with these adjuvants induced dendritic cell maturation in vivo. Also, joint administration of poly(I:C) and anti-CD40 plus HCV antigens had a synergistic effect on the induction of anti-HCV T-cell responses. CD4 responses displayed a Th1 cytokine profile, and CD8 responses could be induced by immunization with a minimal CD8 epitope. Addition of a low amount of NS3 protein (as a source of Th epitopes) to the immunization mixture enhanced CD8 responses, whereas immunization with higher doses of NS3 induced both CD4 and CD8 responses. Surprisingly, immunization with NS3 protein but not with CD8 epitopes was able to induce CD8 responses and able to recognize cells expressing HCV antigens endogenously. Moreover, immunization with these adjuvants activated NK cells, which in turn helped to induce Th1 responses. Finally, this combined immunization protocol afforded long-lasting T-cell responses, suggesting that this strategy may prove to be useful in vaccination and/or treatment of HCV infection.
Permanent link: http://hdl.handle.net/10171/20426
Appears in Collections:DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista

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