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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología hepatitis virales > DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista >

Identification of peptide inhibitors of transforming growth factor beta 1 using a phage-displayed peptide library
Authors: Dotor, J. (Javier)
Lopez-Vazquez, A.B. (Ana B.)
Lasarte, J.J. (Juan José)
Sarobe, P. (Pablo)
Garcia-Granero, M. (Marta)
Riezu-Boj, J.I. (José Ignacio)
Martinez, A. (Alfonso)
Feijoo, E. (Esperanza)
Lopez-Sagaseta, J. (Jacinto)
Hermida, J. (José)
Prieto, J. (Jesús)
Borras-Cuesta, F. (Francisco)
Keywords: Transforming growth factor beta 1
Inhibitor peptides
Phage display
Liver
Fibrosis
Issue Date: 2007
Publisher: Elsevier
Publisher version: http://dx.doi.org/10.1016/j.cyto.2007.06.004
ISSN: 1043-4666
Citation: Dotor J, Lopez-Vazquez AB, Lasarte JJ, Sarobe P, Garcia-Granero M, Riezu-Boj JI, et al. Identification of peptide inhibitors of transforming growth factor beta 1 using a phage-displayed peptide library. Cytokine 2007 Aug;39(2):106-115.
Abstract
Pathologies such as liver fibrosis and scleroderma are characterized by harmful levels of transforming growth factor beta 1 (TGFbeta1). These levels could be neutralized if inhibitors of this cytokine were available. With this aim we searched for peptides with binding affinity for TGFbeta1 using a phage-displayed random 15-mer peptide library. Some peptides thus identified blocked activity of TGFbeta1 in vitro, as measured by their capacity to restore growth of Mv-1-Lu cells in presence of added TGFbeta1. Also, they inhibited TGFbeta1-dependent expression of collagen type I mRNA in liver of mice orally insulted with CCl(4). Intraperitoneal administration of 50 microg of peptide P17 (the most active 15-mer peptide, also referred to as P17(1-15)) inhibited expression of collagen type I mRNA by almost 100%. Interestingly, titration experiments showed that P17(1-12) (a peptide encompassing the first 12 amino acids of P17) was approximately four times more active than P17. These results suggest that both peptides, as well as others reported here, may be of therapeutic interest in processes requiring control of undesired high levels of TGFbeta1.
Permanent link: http://hdl.handle.net/10171/20428
Appears in Collections:DA - CUN - Medicina interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Virología - Artículos de revista
DA - CIMA - Cardiovasculares - Trombosis y Hemostasia - Artículos de Revista
DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista

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