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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología hepatitis virales > DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista >

Vaccination against hepatitis C virus with dendritic cells transduced with an adenovirus encoding NS3 protein
Authors: Zabaleta, A. (Aintzane)
Llopiz, D. (Diana)
Arribillaga, L. (Laura)
Silva, L. (Leyre)
Riezu-Boj, J.I. (José Ignacio)
Lasarte, J.J. (Juan José)
Borras-Cuesta, F. (Francisco)
Prieto, J. (Jesús)
Sarobe, P. (Pablo)
Keywords: Dendritic Cells/transplantation
Hepacivirus/immunology
Transduction, Genetic
Viral Hepatitis Vaccines/administration & dosage
Viral Nonstructural Proteins/genetics
Issue Date: 2008
Publisher: Nature publishing group
Publisher version: http://dx.doi.org/10.1038/sj.mt.6300333
ISSN: 1525-0016
Citation: Zabaleta A, Llopiz D, Arribillaga L, Silva L, Riezu-Boj JI, Lasarte JJ, et al. Vaccination against hepatitis C virus with dendritic cells transduced with an adenovirus encoding NS3 protein. Mol Ther 2008 Jan;16(1):210-217.
Abstract
Chronic infection by hepatitis C virus (HCV) is characterized by the absence of efficient antiviral T-cell responses. Thus, vaccination strategies to induce strong anti-HCV T-cell responses are of paramount importance for prophylactic and therapeutic purposes. Dendritic cells (DCs) are the most potent antigen presenting cells; therefore, immunization with these cells loaded with viral antigens offers a new approach for induction of antiviral immunity. Here we show that immunization with DCs transfected with an adenovirus encoding non-structural 3 protein, from HCV (AdNS3), induced multiepitopic CD4 T helper cell 1 (Th1) and CD8 T-cell responses in different mouse strains. These responses prevented the growth of a tumorexpressing HCV proteins, in short- and long-term experiments. Moreover, immunization with AdNS3-transfected DCs did not induce anti-adenoviral antibodies, as compared to direct immunization with AdNS3, but elicited T-cell responses even in the presence of pre-existing anti-adenoviral antibodies. Finally, responses induced by this protocol down-regulated the expression of HCV RNA in the liver. In conclusion, DCs transfected with AdNS3 may prove to be an efficient anti-HCV vaccine.
Permanent link: http://hdl.handle.net/10171/20430
Appears in Collections:DA - CUN - Medicina interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Virología - Artículos de revista
DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista

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