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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología hepatitis virales > DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista >

Vaccination with an adenoviral vector encoding hepatitis C virus (HCV) NS3 protein protects against infection with HCV-recombinant vaccinia virus
Authors: Arribillaga, L. (Laura)
Lopez-Diaz-de-Cerio, A. (Ascensión)
Sarobe, P. (Pablo)
Casares, N. (Noelia)
Gorraiz, M. (Marta)
Vales, A. (África)
Bruña-Romero, O. (Oscar)
Borras-Cuesta, F. (Francisco)
Paranhos-Baccala, G. (Glaucia)
Prieto, J. (Jesús)
Ruiz, J. (Juan)
Lasarte, J.J. (Juan José)
Keywords: Recombinant adenovirus
Hepatitis C virus
In vivo protection
Issue Date: 2002
Publisher: Elsevier
Publisher version: http://dx.doi.org/10.1016/S0264-410X(02)00456-5
ISSN: 0264-410X
Citation: Arribillaga L, de Cerio AL, Sarobe P, Casares N, Gorraiz M, Vales A, et al. Vaccination with an adenoviral vector encoding hepatitis C virus (HCV) NS3 protein protects against infection with HCV-recombinant vaccinia virus. Vaccine 2002 Dec 13;21(3-4):202-210.
Abstract
Cellular immune response plays an important role in the clearance of hepatitis C virus (HCV). Thus, development of efficient ways to induce anti-viral cellular immune responses is an important step toward prevention and/or treatment of HCV infection. With this aim, we have constructed a replication-deficient recombinant adenovirus expressing HCV NS3 protein (RAdNS3). The efficacy of RAdNS3 was tested in vivo by measuring the protection against infection with a recombinant vaccinia virus expressing HCV-polyprotein (vHCV1-3011). Immunisation with 10(9)pfu of RAdNS3 induced anti-NS3 humoral, T helper and T cytotoxic responses. We identified eight epitopes recognised by IFN-gamma producing cells, five of them exhibiting lytic activity. Moreover, we show that RAdNS3 immunised mice were protected against challenge with vHCV1-3011 and that this protection was mediated by CD8(+) cells. In conclusion, our results suggest that adenoviral vectors encoding NS3 might be useful for the induction of prophylactic and/or therapeutic anti-HCV immunity.
Permanent link: http://hdl.handle.net/10171/20615
Appears in Collections:DA - CIMA - Oncología - Inmunoterapia - Artículos de Revista
DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista

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