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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología hepatitis virales > DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista >

Protection against woodchuck hepatitis virus (WHV) infection by gene gun coimmunization with WHV core and interleukin-12
Authors: Garcia-Navarro, R. (Raquel)
Blanco-Urgoiti, B. (Begoña)
Berraondo, P. (Pedro)
Sanchez-de-la-Rosa, R. (R.)
Vales, A. (África)
Hervas-Stubbs, S. (Sandra)
Lasarte, J.J. (Juan José)
Borras-Cuesta, F. (Francisco)
Ruiz, J. (Juan)
Prieto, J. (Jesús)
Keywords: Hepatitis B/immunology
Hepatitis B/prevention & control
Hepatitis B Virus, Woodchuck/immunology
Interleukin-12/immunology
Issue Date: 2001
Publisher: American Society for Microbiology
Publisher version: http://jvi.asm.org/content/75/19/9068.full
ISSN: 0022-538X
Citation: Garcia-Navarro R, Blanco-Urgoiti B, Berraondo P, Sanchez de la Rosa R, Vales A, Hervas-Stubbs S, et al. Protection against woodchuck hepatitis virus (WHV) infection by gene gun coimmunization with WHV core and interleukin-12. J Virol 2001 Oct;75(19):9068-9076.
Abstract
Woodchuck hepatitis virus (WHV) and hepatitis B virus (HBV) are closely similar with respect to genomic organization, host antiviral responses, and pathobiology of the infection. T-cell immunity against viral nucleocapsid (HBcAg or WHcAg) has been shown to play a critical role in viral clearance and protection against infection. Here we show that vaccination of healthy woodchucks by gene gun bombardment with a plasmid coding for WHcAg (pCw) stimulates proliferation of WHcAg-specific T cells but that these cells do not produce significant levels of gamma interferon (IFN-gamma) upon antigen stimulation. In addition, animals vaccinated with pCw alone were not protected against WHV inoculation. In order to induce a Th1 cytokine response, another group of woodchucks was immunized with pCw together with another plasmid coding for woodchuck interleukin-12 (IL-12). These animals exhibited WHcAg-specific T-cell proliferation with high IFN-gamma production and were protected against challenge with WHV, showing no viremia or low-level transient viremia after WHV inoculation. In conclusion, gene gun immunization with WHV core generates a non-Th1 type of response which does not protect against experimental infection. However, steering the immune response to a Th1 cytokine profile by IL-12 coadministration achieves protective immunity. These data demonstrate a crucial role of Th1 responses in the control of hepadnavirus replication and suggest new approaches to inducing protection against HBV infection.
Permanent link: http://hdl.handle.net/10171/20619
Appears in Collections:DA - CIMA - Terapia génica y Hepatología - Terapia génica del cáncer - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología terapia génica - Artículos de revista
DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista

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