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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología hepatitis virales > DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista >

Abnormal priming of CD4(+) T cells by dendritic cells expressing hepatitis C virus core and E1 proteins
Authors: Sarobe, P. (Pablo)
Lasarte, J.J. (Juan José)
Casares, N. (Noelia)
Lopez-Diaz-de-Cerio, A. (Ascensión)
Baixeras, E. (Elena)
Labarga, P. (Pablo)
Garcia, N. (Nicolás)
Borras-Cuesta, F. (Francisco)
Prieto, J. (Jesús)
Keywords: CD4-Positive T-Lymphocytes/immunology
Dendritic Cells/immunology
Hepacivirus/immunology
Hepatitis C, Chronic/immunology
Issue Date: 2002
Publisher: American Society for Microbiology
Publisher version: http://dx.doi.org/10.1128/JVI.76.10.5062-5070.2002
ISSN: 0022-538X
Citation: Sarobe P, Lasarte JJ, Casares N, Lopez-Diaz de Cerio A, Baixeras E, Labarga P, et al. Abnormal priming of CD4(+) T cells by dendritic cells expressing hepatitis C virus core and E1 proteins. J Virol 2002 May;76(10):5062-5070.
Abstract
Patients infected with hepatitis C virus (HCV) have an impaired response against HCV antigens while keeping immune competence for other antigens. We hypothesized that expression of HCV proteins in infected dendritic cells (DC) might impair their antigen-presenting function, leading to a defective anti-HCV T-cell immunity. To test this hypothesis, DC from normal donors were transduced with an adenovirus coding for HCV core and E1 proteins and these cells (DC-CE1) were used to stimulate T lymphocytes. DC-CE1 were poor stimulators of allogeneic reactions and of autologous primary and secondary proliferative responses. Autologous T cells stimulated with DC-CE1 exhibited a pattern of incomplete activation characterized by enhanced CD25 expression but reduced interleukin 2 production. The same pattern of incomplete lymphocyte activation was observed in CD4(+) T cells responding to HCV core in patients with chronic HCV infection. However, CD4(+) response to HCV core was normal in patients who cleared HCV after alpha interferon therapy. Moreover, a normal CD4(+) response to tetanus toxoid was found in both chronic HCV carriers and patients who had eliminated the infection. Our results suggest that expression of HCV structural antigens in infected DC disturbs their antigen-presenting function, leading to incomplete activation of anti-HCV-specific T cells and chronicity of infection. However, presentation of unrelated antigens by noninfected DC would allow normal T-cell immunity to other pathogens.
Permanent link: http://hdl.handle.net/10171/20620
Appears in Collections:DA - CIMA - Oncología - Inmunoterapia - Artículos de Revista
DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista

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