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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Ciencias Cardiovasculares > Trombosis y hemostasia > DA - CIMA - Cardiovasculares - Trombosis y Hemostasia - Artículos de Revista >

sPLA2-V inhibits EPCR anticoagulant and antiapoptotic properties by accommodating lysophosphatidylcholine or PAF in the hydrophobic groove
Authors: Lopez-Sagaseta, J. (Jacinto)
Puy, C. (Cristina)
Tamayo, I. (Ibai)
Allende, M. (Mikel)
Cervero, J. (Jorge)
Velasco, S.E. (Susana E.)
Esmon, C.T. (Charles T.)
Montes, R. (Ramón)
Hermida, J. (José)
Keywords: Endothelial cell protein C receptor
Secretory group V phospholipase A2
Phosphatidylcholine
Lysophosphatidylcholine
Platelet activating factor
Issue Date: 2012
Publisher: American Society of Hematology
Publisher version: http://bloodjournal.hematologylibrary.org/content/early/2011/12/13/blood-2011-05-353409
ISSN: 1528-0020
Citation: Lopez-Sagaseta J, Puy C, Tamayo I, Allende M, Cervero J, Velasco SE, et al. sPLA2-V inhibits EPCR anticoagulant and antiapoptotic properties by accommodating lysophosphatidylcholine or PAF in the hydrophobic groove. Blood 2012 Mar 22;119(12):2914-2921.
Abstract
The endothelial protein C receptor (EPCR) plays an important role in cardiovascular disease by binding protein C/activated protein C (APC). EPCR structure contains a hydrophobic groove filled with an unknown phospholipid needed to perform its function. It has not been established whether lipid exchange takes place in EPCR as a regulatory mechanism of its activity. Our objective was to identify this phospholipid and to explore the possibility of lipid exchange as a regulatory mechanism of EPCR activity driven by the endothelially expressed secretory group V phospholipase A2 (sPLA2-V). We identified phosphatidylcholine (PCh) as the major phospholipid bound to human soluble EPCR (sEPCR). PCh in EPCR could be exchanged for lysophosphatidylcholine (lysoPCh) and platelet activating factor (PAF). Remarkably, lysoPCh and PAF impaired the protein C binding ability of sEPCR. Inhibition of sPLA2-V, responsible for lysoPCh and PAF generation, improved APC binding to endothelial cells. EPCR-dependent protein C activation and APC antiapoptotic effect were thus significantly enhanced. In contrast, endothelial cell supplementation with sPLA2-V inhibited both APC generation and its antiapoptotic effects. We conclude that APC generation and function can be modulated by changes in phospholipid occupancy of its endothelial cell receptor.
Permanent link: http://hdl.handle.net/10171/21133
Appears in Collections:DA - CIMA - Cardiovasculares - Trombosis y Hemostasia - Artículos de Revista

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