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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Hepatología bioquímica > DA - CIMA - Terapia génica y Hepatología - Hepatología bioquímica - Artículos de revista >

S-Nitrosation of proteins during D-galactosamine-induced cell death in human hepatocytes
Authors: Lopez-Sanchez, L.M. (Laura M.)
Collado, J.A. (Juan A.)
Corrales, F.J. (Fernando José)
Lopez-Cillero, P. (Pedro)
Montero, J.L. (José L.)
Fraga, E. (Enrique)
Serrano, J. (Juan)
Mata, M. (Manuel) de la
Muntane, J. (Jordi)
Rodriguez-Ariza, A. (Antonio)
Keywords: Hepatocyte
d-Galactosamine
Apoptosis
Necrosis
S-nitrosation
Proteomics
Issue Date: 2007
Publisher: Informa Healthcare
Publisher version: http://informahealthcare.com/doi/abs/10.1080/10715760600943918
ISSN: 1029-2470
Citation: Lopez-Sanchez LM, Collado JA, Corrales FJ, Lopez-Cillero P, Montero JL, Fraga E, et al. S-Nitrosation of proteins during D-galactosamine-induced cell death in human hepatocytes. Free Radic Res 2007 Jan;41(1):50-61.
Abstract
Nitric oxide (NO) participates in the cell death induced by d-Galactosamine (d-GalN) in hepatocytes, and NO-derived reactive oxygen intermediates are critical contributors to protein modification and hepatocellular injury. It is anticipated that S-nitrosation of proteins will participate in the mechanisms leading to cell death in d-GalN-treated human hepatocytes. In the present study, d-GalN-induced cell death was related to augmented levels of NO production and S-nitrosothiol (SNO) content. The biotin switch assay confirmed that d-GalN increased the levels of S-nitrosated proteins in human hepatocytes. S-nitrosocysteine (CSNO) enhanced protein S-nitrosation and altered cell death parameters that were related to S-nitrosation of the executioner caspase-3. Fifteen S-nitrosated proteins participating in metabolism, antioxidative defense and cellular homeostasis were identified in human hepatocytes treated with CSNO. Among them, seven were also identified in d-GalN-treated hepatocytes. The results here reported underline the importance of the alteration of SNO homeostasis during d-GalN-induced cell death in human hepatocytes
Permanent link: http://hdl.handle.net/10171/21476
Appears in Collections:DA - CIMA - Unidad de Proteómica, Genómica y Bioinformática - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Hepatología bioquímica - Artículos de revista

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