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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Hepatología bioquímica > DA - CIMA - Terapia génica y Hepatología - Hepatología bioquímica - Artículos de revista >

Mass spectrometric characterization of mitochondrial complex I NDUFA10 variants
Authors: Muñoz, J. (Javier)
Fernandez-Irigoyen, J. (Joaquín)
Santamaria, E. (Enrique)
Axel, P. (Parbel)
Obeso, J.A. (José A.)
Corrales, F.J. (Fernando José)
Keywords: Mass spectrometry
Mitochondria
NDUFA10
Posttranslational modifications
Issue Date: 2008
Publisher: Wiley-VCH Verlag Berlin
Publisher version: http://onlinelibrary.wiley.com/doi/10.1002/pmic.200701085/abstract
ISSN: 1615-9861
Citation: Munoz J, Fernandez-Irigoyen J, Santamaria E, Parbel A, Obeso J, Corrales FJ. Mass spectrometric characterization of mitochondrial complex I NDUFA10 variants. Proteomics 2008 May;8(9):1898-1908.
Abstract
In the present study, we have used a combination of 2-DE and MS to isolate and characterize two variants of the mitochondrial complex I subunit NDUFA10 from Wistar rat brain. Extensive MS/MS analysis revealed that a D/N substitution at position 120 resulting from a 353A/G transition in the coding gene is the biochemical difference between the two most abundant NDUFA10 isoforms. Moreover, 33 modifications of distinct chemical nature targeting 59 specific residues were found to be common to the acidic and basic forms. Positions C67, H149 and H322 of NDUFA10 were specially targeted by different modifications suggesting the high reactivity of these residues and their potential implication in the regulation of the protein function. Together with nonenzymatic modifications that can form in the sample isolation and workup steps, such as oxidation of methionine, tryptophan, cysteine and histidine, we describe amino acid variants of unknown chemical structure that must be further characterized, as well as accumulation of R, K and H methylations and probably K acetylations at the C-terminal region that might play a role in the control of NDUFA10 activity according to similar mechanisms to those described for histones.
Permanent link: http://hdl.handle.net/10171/21523
Appears in Collections:DA - CIMA - Unidad de Proteómica, Genómica y Bioinformática - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Hepatología bioquímica - Artículos de revista

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