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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Hepatología bioquímica > DA - CIMA - Terapia génica y Hepatología - Hepatología bioquímica - Artículos de revista >

Molecular profiling of hepatocellular carcinoma in mice with a chronic deficiency of hepatic s-adenosylmethionine: relevance in human liver diseases
Authors: Santamaria, E. (Enrique)
Muñoz, J. (Javier)
Fernandez-Irigoyen, J. (Joaquín)
Sesma, L. (Laura)
Mora, M.I. (María I.)
Berasain, C. (Carmen)
Lu, S.C. (Shelly C.)
Mato, J.M. (José María)
Prieto, J. (Jesús)
Avila, M.A. (Matías Antonio)
Corrales, F.J. (Fernando José)
Keywords: S-adenosylmethionine
Hepatocellular carcinoma
Two-dimensional gel electrophoresis
Mass spectrometry
Proteomics
Biomarkers
Issue Date: 2006
Publisher: American Chemical Society
Publisher version: http://pubs.acs.org/doi/abs/10.1021/pr050429v
ISSN: 1535-3907
Citation: Santamaria E, Munoz J, Fernandez-Irigoyen J, Sesma L, Mora MI, Berasain C, et al. Molecular profiling of hepatocellular carcinoma in mice with a chronic deficiency of hepatic s-adenosylmethionine: relevance in human liver diseases. J Proteome Res 2006 Apr;5(4):944-953.
Abstract
S-adenosylmethionine arises as a central molecule in the preservation of liver homeostasis as a chronic hepatic deficiency results in spontaneous development of steatohepatitis and hepatocellular carcinoma. In the present work, we have attempted a comprehensive analysis of proteins associated with hepatocarcinogenesis in MAT1A knock out mice using a combination of two-dimensional electrophoresis and mass spectrometry, to then apply the resulting information to identify hallmarks of human HCC. Our results suggest the existence of individual-specific factors that might condition the development of preneoplastic lesions. Proteomic analysis allowed the identification of 151 differential proteins in MAT1A-/- mice tumors. Among all differential proteins, 27 changed in at least 50% of the analyzed tumors, and some of these alterations were already detected months before the development of HCC in the KO liver. The expression level of genes coding for 13 of these proteins was markedly decreased in human HCC. Interestingly, seven of these genes were also found to be down-regulated in a pretumoral condition such as cirrhosis, while depletion of only one marker was assessed in less severe liver disorders.
Permanent link: http://hdl.handle.net/10171/21560
Appears in Collections:DA - CIMA - Terapia génica y Hepatología - Oncobiología - Artículos de revista
DA - CIMA - Unidad de Proteómica, Genómica y Bioinformática - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Hepatología bioquímica - Artículos de revista

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