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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología experimental > DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista >

T(h)1 but not T(h)0 cell help is efficient to induce cytotoxic T lymphocytes by immunization with short synthetic peptides
Authors: Lopez-Diaz-de-Cerio, A. (Ascensión)
Casares, N. (Noelia)
Lasarte, J.J. (Juan José)
Sarobe, P. (Pablo)
Perez-Mediavilla, L.A. (Luis Alberto)
Ruiz, M. (Marta)
Prieto, J. (Jesús)
Borras-Cuesta, F. (Francisco)
Keywords: Cytotoxic T lymphocyte induction
T cell help
Cytokines
Th determinant
Issue Date: 1999
Publisher: Oxford University Press
Publisher version: http://intimm.oxfordjournals.org/content/11/12/2025
ISSN: 1460-2377
Citation: Lopez-Diaz de Cerio AL, Casares N, Lasarte JJ, Sarobe P, Perez-Mediavilla LA, Ruiz M, et al. T(h)1 but not T(h)0 cell help is efficient to induce cytotoxic T lymphocytes by immunization with short synthetic peptides. Int Immunol 1999 Dec;11(12):2025-2034.
Abstract
Immunization of BALB/c mice with peptide HVSGHRMAWDMMMNWA, encompassing residues 121-135 from hepatitis C virus E1 protein, induced CD4(+) T(h)1 cells as well as a long-lasting CD8(+) cytotoxic T lymphocyte (CTL) response in vivo when the peptide was administered s.c. with or without incomplete Freund's adjuvant. Using truncated peptides from this sequence it was shown that the determinant recognized by cytotoxic T cells was encompassed by residues SGHRMAWDM. Deletion of residues from the N-terminus or the C-terminus of the wild-type peptide abrogated its helper character. When Val122 of the wild peptide was replaced by Ala, the ability to induce a cytotoxic response was lost concomitantly with the loss of the T(h)1 pattern of cytokine production. Interestingly, the Ala-modified peptide, when co-immunized with a peptide encompassing residues 323-329 from ovalbumin (OVA), which is able to induce a T(h)1 response in BALB/c mice, restored the capacity of the modified peptide to induce CTL. However, co-immunization of the Ala-modified peptide with a peptide encompassing residues 106-118 from sperm whale myoglobin, which induces a T(h)0 cytokine profile in BALB/c mice, was much less efficient than the OVA peptide to restore CTL induction. These results demonstrate that CTL induction with a short synthetic peptide requires that this peptide contains domains recognized by T(c) cells as well as by T(h)1 cells. For those peptides that do not contain this type of T(h) domain, competent T cell help can be provided by co-immunization with a distinct peptide that is able to stimulate a T(h)1 response.
Permanent link: http://hdl.handle.net/10171/21650
Appears in Collections:DA - CIMA - Oncología - Inmunoterapia - Artículos de Revista
DA - CIMA - Neurociencias - Neurofarmacología y conducta - Artículos de Revista
DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista

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