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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología hepatitis virales > DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista >

Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus
Authors: Bruña-Romero, O. (Oscar)
Lasarte, J.J. (Juan José)
Wilkinson, G. (Gavin)
Grace, K. (Ken)
Klarke, B. (Berwyn)
Borras-Cuesta, F. (Francisco)
Prieto, J. (Jesús)
Keywords: Defective Viruses/immunology
Hepacivirus/immunology
T-Lymphocytes, Cytotoxic/immunology
Viral Core Proteins/immunology
Viral Envelope Proteins/immunology
Issue Date: 1997
Publisher: Wiley-Blackwell
Publisher version: http://onlinelibrary.wiley.com/doi/10.1002/hep.510250236/abstract
ISSN: 1527-3350
Citation: Bruna-Romero O, Lasarte JJ, Wilkinson G, Grace K, Clarke B, Borras-Cuesta F, et al. Induction of cytotoxic T-cell response against hepatitis C virus structural antigens using a defective recombinant adenovirus. Hepatology 1997 Feb;25(2):470-477.
Abstract
A replication-defective recombinant adenovirus (RAd), RAdCMV-CE1, containing core and E1 genes of hepatitis C virus (HCV) was constructed. RAdCMV-CE1 was able to express core and E1 proteins both in mice and human cells. Immunization of BALB/c mice with RAdCMV-CE1 induced a specific cytotoxic T-cell response against the two HCV proteins. This response was characterized using a panel of 60 synthetic 14- or 15-mer overlapping peptides (10 amino-acid overlap) spanning the entire sequence of these proteins. Five main epitopes were found in the core protein, four of which had been previously described either in mice or humans. One single novel epitope was found in E1. Fine mapping of this E1 determinant, showed that octamer GHRMAWDM is the minimal epitope recognized by cytotoxic T lymphocytes (CTL). The cytotoxic T-cell response was H-2d restricted, lasted for at least 100 days, and was mediated by T cells with the classic CD4-CD8+ phenotype. This work demonstrates that replication-defective recombinant adenoviruses can efficiently express HCV proteins and are able to induce an in vivo cytotoxic T-cell response against a diversity of epitopes from HCV antigens. These vectors should be taken into consideration in the design of vaccines and also as a means to stimulate specific T-cell responses in chronic HCV carriers.
Permanent link: http://hdl.handle.net/10171/21662
Appears in Collections:DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista

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