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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología experimental > DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista >

Further insights on the inhibition of HIV type 1 infection in vitro by CD4-modified synthetic peptides containing phenylalanine
Authors: Prieto, I. (Isidro)
Lasarte, J.J. (Juan José)
Sarobe, P. (Pablo)
Golvano, J.J. (José Javier)
Civeira, M.P. (María Pilar)
Gullon, A. (Arturo)
Prieto, J. (Jesús)
Borras-Cuesta, F. (Francisco)
Keywords: Acquired Immunodeficiency Syndrome/prevention & control
Antigens, CD4/pharmacology
Antiviral Agents/pharmacology
HIV-1/pathogenicity
Oligopeptides/pharmacology
Peptide Fragments/pharmacology
Phenylalanine
Issue Date: 1996
Publisher: Mary Ann Liebert
Publisher version: http://online.liebertpub.com/doi/abs/10.1089/aid.1996.12.1023
ISSN: 1931-8405
Citation: Prieto I, Lasarte JJ, Sarobe P, Golvano J, Civeira MP, Gullon A, et al. Further insights on the inhibition of HIV type 1 infection in vitro by CD4-modified synthetic peptides containing phenylalanine. AIDS Res Hum Retroviruses 1996 Jul 20;12(11):1023-1030.
Abstract
Phenylalanine-containing peptides from CD4 were synthesized on the basis of chemical similarity with active CD4(81-92)-benzylated peptides. Systematic replacement of amino acids of these peptides bearing the benzyl group by phenylalanine, afforded several peptides that were able to block the binding of gp120 to CD4 and to inhibit HIV-induced syncytium formation. These experiments showed that substitution of residues 81 and 85 by phenylalanine was the most important for activity. Following optimization of the length of phenylalanine-substituted peptides it was found that FYICFVED and FYICFVEDE were the most active. Their IC50 for the inhibition of syncytium formation was around 1.2-1.6 microM. This activity is at least 30 times higher than that of the parent peptide FYIFFVEDQKEEDD previously reported (Lasarte et al., J Acquir Immune Defic Syndr 1994;7:129-134). Binding competition experiments with two different anti-peptide antisera recognizing the V3 region of gp120 and FYICFVEDE, show that the active peptides bind to V3 or to a sterically near region of V3. None of the active peptides was toxic to cells in vitro. The enhanced activity and simplicity of these new phenylalanine-substituted CD4 peptides might be a good starting point for the development of mimotopes of potential use for the treatment of AIDS.
Permanent link: http://hdl.handle.net/10171/21668
Appears in Collections:DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista

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