Dadun/></a>
				</td>
				<td class= Dadun
   (New user)
Help  | Contact  |  Castellano English  
 

Dadun > Depósito Académico > Facultad de Farmacia > Departamento de Ciencias de la Alimentación, Fisiología y Toxicología > DA - Farmacia - CAFT - Artículos de revista >

Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
Authors: Menendez-Carreño, M. (María)
Garcia-Herreros, C. (Cecilia)
Ansorena, D. (Diana)
Astiasaran, I. (Iciar)
Keywords: Serum
Sterols
COPs
Linearity
Recovery
Repeatability
Issue Date: 2008
Publisher: Elsevier
Publisher version: http://dx.doi.org/10.1016/j.jchromb.2008.01.036
ISSN: 1570-0232
Citation: Menendez-Carreno M, Garcia-Herreros C, Astiasaran I, Ansorena D. Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 15;864(1-2):61-68.
Abstract
A validated gas chromatography-mass spectrometry (GC-MS) detection method for the quantitative analysis of sterol oxidation products (SOPs) in serum is described. After a lipid extraction procedure with chloroform-methanol, a cold saponification and purification by solid phase extraction, oxysterols were derivatized to form trimethyl-sylil-ethers which were subjected to GC-MS analysis. Calibration curves for cholesterol oxidation products showed determination coefficient (R(2)) of 1.0, with low values for the coefficient of variation of the response factors (< 1%). Detection and quantification limits were below 5 ng/mL and 10 ng/mL, respectively. Recovery data were between 77.65% and 110.29% (CV < 10% for all compounds). Good results were obtained for within- and between-day repeatability, with values below 10%. In conclusion, the method performed is suitable for the determination and quantification of SOPs in serum.
Permanent link: http://hdl.handle.net/10171/22124
Appears in Collections:DA - Farmacia - CAFT - Artículos de revista

Files in This Item:
File:  amended manuscript II maria validacion.pdf
Description: 
Size:  249,77 kB
Format:  Adobe PDF
 View / Open 

Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.