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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Ciencias Cardiovasculares > Aterosclerosis e inflamación > DA - CIMA - Cardiovasculares - Aterosclerosis e inflamación - Artículos de revista >

Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
Authors: Orbe, J. (Josune)
Montes, R. (Ramón)
Zabalegui, N. (Natalia)
Perez-Ruiz, A. (Ana)
Paramo, J.A. (José Antonio)
Keywords: Heparin
mRNA
Hirudin
Endotoxin
PAI-1
HUVEC
Issue Date: 1999
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S0049384898002035
ISSN: 1879-2472
Citation: Orbe J, Montes R, Zabalegui N, Perez-Ruiz A, Paramo JA. Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells. Thromb Res 1999 May 1;94(3):137-145.
Abstract
Heparin and other antithrombotic drugs besides their anticoagulant action could have a profibrinolytic effect. We have analyzed the effect of unfractionated heparin (UFH) and hirudin on PAI-1 gene expression in human umbilical vein endothelial cells (HUVEC). Cells were stimulated with UFH (1 and 10 IU/ml) and hirudin (20 and 100 TIU/ml). Samples were obtained before and 2, 6, and 24 hours after stimulation. mRNA analysis was conducted by reverse transcription followed by polymerase chain reaction, and PAI-1 antigen was determined by ELISA. Addition of UFH (10 IU/ml) to HUVEC resulted in a decrease of PAI-1 mRNA at 6 hours (40% reduction) and 24 hours (60% reduction) and PAI-1 antigen. Hirudin, however, did not modify significantly the PAI-1 mRNA nor the inhibitor secretion. The addition of UFH (10 or 100 IU/ml) to endotoxin-stimulated HUVEC also reduced the increased PAI-1 mRNA and antigen secretion (45%), whereas no effect could be observed with hirudin. Our results suggest that UFH, but not hirudin, by reducing the endothelial expression of PAI-1 might have a profibrinolytic effect.
Permanent link: http://hdl.handle.net/10171/22262
Appears in Collections:DA - CIMA - Cardiovasculares - Trombosis y Hemostasia - Artículos de Revista
DA - CIMA - Cardiovasculares - Aterosclerosis e inflamación - Artículos de revista

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