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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Ciencias Cardiovasculares > Aterosclerosis e inflamación > DA - CIMA - Cardiovasculares - Aterosclerosis e inflamación - Artículos de revista >

Dietary supplementation with vitamins C and E prevents downregulation of endothelial NOS expression in hypercholesterolemia in vivo and in vitro
Authors: Rodriguez, J.A. (José Antonio)
Grau, A. (Andrés)
Eguinoa, E. (Ezequiel)
Nespereira, B. (Beatriz)
Perez-Ilzarbe, M. (Maitane)
Arias, R. (Roberto)
Belzunce, M.S. (María S.)
Paramo, J.A. (José Antonio)
Martinez-Caro, D. (Diego)
Keywords: Atherosclerosis
Endothelium
Lipoproteins
Nitric oxide
Tocopherol
Swine
Issue Date: 2002
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S0021915002001880
ISSN: 1879-1484
Citation: Rodriguez JA, Grau A, Eguinoa E, Nespereira B, Perez-Ilzarbe M, Arias R, et al. Dietary supplementation with vitamins C and E prevents downregulation of endothelial NOS expression in hypercholesterolemia in vivo and in vitro. Atherosclerosis 2002 Nov;165(1):33-40.
Abstract
Impaired endothelium-dependent vasodilation has been associated with decreased NO bioavailability in hypercholesterolemia. This study aimed to determine whether antioxidant vitamins C and E could improve hypercholesterolemia-derived endothelial dysfunction in the porcine model, and whether observed in vivo results could be reproduced in vitro by incubation of coronary endothelial cells (EC) in the presence of native low-density lipoproteins (LDL). Adult mini-pigs were fed standard (C), cholesterol rich (HC) or cholesterol rich diet supplemented with vitamins C and E (HCV). Endothelium-dependent blood flow increase in response to acetylcholine was determined. Endothelial nitric oxide synthase (eNOS) expression was measured in arterial samples and in EC incubated with LDL isolated from porcine plasma. Vasomotor response to acetylcholine in HC was significantly lower (P<0.05) than control and HCV. There was a significant (P<0.05) decrease in eNOS immunoreactivity in HC, compared with HCV and control. Native LDL from HC, but not from HCV, induced a significant decrease in eNOS expression. Vitamins C and E treatment improved the endothelium-dependent vasomotor capacity and prevented decreased expression of eNOS in hypercholesterolemic pigs. A similar effect could be demonstrated in vitro, by incubation of EC with native LDL, suggesting that the effect of physiologically-modified LDL on eNOS could have a role in recovering vascular function.
Permanent link: http://hdl.handle.net/10171/22272
Appears in Collections:DA - CIMA - Cardiovasculares - Aterosclerosis e inflamación - Artículos de revista

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