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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Ciencias Cardiovasculares > Aterosclerosis e inflamación > DA - CIMA - Cardiovasculares - Aterosclerosis e inflamación - Artículos de revista >

Purification and characterization of a variant of human prothrombin: prothrombin Segovia
Authors: Collados, M.T. (María T.)
Fernandez, J. (Javier)
Paramo, J.A. (José Antonio)
Montes, R. (Ramón)
Borbolla, J.R. (José R.)
Montaño, L.F. (Luis F.)
Rocha, E. (Eduardo)
Keywords: Dysprothrombinemia
Prothrombin activation
Prothrombinasa complex
Issue Date: 1997
Publisher: Elsevier
Publisher version: http://www.sciencedirect.com/science/article/pii/S0049384897000364
ISSN: 1879-2472
Citation: Collados MT, Fernandez J, Paramo JA, Montes R, Borbolla JR, Montano LF, et al. Purification and characterization of a variant of human prothrombin: prothrombin Segovia. Thromb Res 1997 Mar 15;85(6):465-477.
Abstract
A dysprothrombin designated prothrombin Segovia was isolated from the plasma of an individual with normal prothrombin antigen and prothrombin activity lesser than 25% of the control prothrombin activity. Activation by prothrombinase complex showed a lower amidolytic than clotting activity, which suggests a lesser generation of active intermediates than normal prothrombin. When prothrombin Segovia was activated by prothrombinase complex in the absence of factor Va, no thrombin formation was found by functional activities. SDS-PAGE analysis of the molecules derived by activation with prothrombinase complex, Taipan snake venom and Echis carinatus venom showed an accumulation of molecules not cleaved at bond Arg320-Ile321. This was more evident with Echis carinatus venom, which only acts on this bond. Our data suggest that the alteration of prothrombin Segovia impairs the scission of bond Arg320-Ile321.
Permanent link: http://hdl.handle.net/10171/22334
Appears in Collections:DA - CIMA - Cardiovasculares - Trombosis y Hemostasia - Artículos de Revista
DA - CUN - Hematología y Hemoterapia - Artículos de revista
DA - CIMA - Cardiovasculares - Aterosclerosis e inflamación - Artículos de revista

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