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Dadun > Depósito Académico > Facultad de Farmacia > Departamento de Farmacia y Tecnología Farmacéutica > DA - Farmacia - Tecnología Farmacéutica - Artículos de revista >

Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies.
Authors: Echevarría, L. (Lydia)
Blanco-Prieto, M.J. (María José)
Campanero, M.A. (Miguel Angel)
Santoyo, S. (Susana)
Keywords: Skin recovery
Mupirocin
Issue Date: Nov-2003
Publisher: Elsevier
Publisher version: http://dx.doi.org/10.1016/j.jchromb.2003.07.011
ISSN: 1570-0232
Citation: Echevarria L, Blanco-Prieto MJ, Campanero MA, Santoyo S, Ygartua P. Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. J Chromatogr B Analyt Technol Biomed Life Sci 2003 Nov 5;796(2):233-241.
Abstract
A simple, rapid, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) method for the measurement of mupirocin concentrations in both skin layers and percutaneous samples has been developed. Mupirocin was extracted from skin layers using PBS–acetonitrile (90:10, v/v). The method is sufficiently sensitive and repeatable to be used in percutaneous penetration studies. The samples were chromatographed on a View the MathML source mm C8 LiChrospher Select B (5 μm). The mobile phase composition was a mixture of acetonitrile–ammonium acetate 0.05 M (27.5:72.5, v/v) adjusted to pH 6.3 with acetic acid. The analyte was detected at 228 nm and the run time was 11 min. Linearity was confirmed in the concentration range 0.2–20 μg/ml and the limit of detection was 9.5 ng/ml.
Permanent link: http://hdl.handle.net/10171/22388
Appears in Collections:DA - CUN - Farmacología clínica - Artículos de revista
DA - Farmacia - Tecnología Farmacéutica - Artículos de revista

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