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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Vectores > DA - CIMA - Terapia génica y Hepatología - Vectores - Artículos de revista >

Combination of RNA interference and U1 inhibition leads to increased inhibition of gene expression
Authors: Abad, X. (Xabier)
Razquin, N. (Nerea)
Abad, A. (Amaya)
Fortes, P. (Puri)
Keywords: RNA Interference
RNA, Small Nuclear/antagonists & inhibitors
Issue Date: 2010
Publisher: Oxford University Press
Publisher version: http://nar.oxfordjournals.org/content/38/13/e136
ISSN: 1362-4962
Citation: Abad X, Razquin N, Abad A, Fortes P. Combination of RNA interference and U1 inhibition leads to increased inhibition of gene expression. Nucleic Acids Res 2010 Jul;38(13):e136.
Abstract
RNA interference (RNAi) has been revolutionary for the specific inhibition of gene expression. However, the application of RNAi has been hampered by the fact that many siRNAs induce dose-dependent unwanted secondary effects. Therefore, new methods to increase inhibition of gene expression with low doses of inhibitors are required. We have tested the combination of RNAi and U1i (U1 small nuclear RNA--snRNA--interference). U1i is based on U1 inhibitors (U1in), U1 snRNA molecules modified to target a pre-mRNA and inhibit its gene expression by blocking nuclear polyadenylation. The combination of RNAi and U1i resulted in stronger inhibition of reporter or endogenous genes than that obtained using either of the techniques alone. The increased inhibition observed is stable over time and allows higher inhibition than the best obtained with either of the inhibitors alone even with decreased doses of the inhibitors. We believe that the combination of RNAi and U1i will be of interest when higher inhibition is required or when potent inhibitors are not available. Also, the combination of these techniques would allow functional inhibition with a decreased dose of inhibitors, avoiding toxicity due to dose-dependent unwanted effects.
Permanent link: http://hdl.handle.net/10171/23170
Appears in Collections:DA - CIMA - Terapia génica y Hepatología - Vectores - Artículos de revista

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