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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Hepatología experimental > DA - CIMA - Terapia génica y Hepatología - Hepatología experimental - Artículos de revista >

Effect of ursodeoxycholic acid on methionine adenosyltransferase activity and hepatic glutathione metabolism in rats
Authors: Rodriguez-Ortigosa, C.M. (Carlos M.)
Cincu, R.N. (Rafael N.)
Sanz, S. (S.)
Ruiz, F. (F.)
Quiroga, J. (Jorge)
Prieto, J. (Jesús)
Keywords: Glutathione/metabolism
Liver/metabolism
Methionine Adenosyltransferase/drug effects
Ursodeoxycholic Acid/pharmacology
Issue Date: 2002
Publisher: BMJ Publishing Group
Publisher version: http://gut.bmj.com/content/50/5/701
ISSN: 1468-3288
Citation: Rodriguez-Ortigosa CM, Cincu RN, Sanz S, Ruiz F, Quiroga J, Prieto J. Effect of ursodeoxycholic acid on methionine adenosyltransferase activity and hepatic glutathione metabolism in rats. Gut 2002 May;50(5):701-706.
Abstract
BACKGROUND AND AIMS: Both bile salts and glutathione participate in the generation of canalicular bile flow. In this work, we have investigated the effect of different bile salts on hepatic glutathione metabolism. METHODS: Using the isolated and perfused rat liver, we studied hepatic glutathione content, and metabolism and catabolism of this compound in livers perfused with taurocholate, ursodeoxycholate, or deoxycholate. RESULTS: We found that in livers perfused with ursodeoxycholate, levels of glutathione and the activity of methionine adenosyltransferase (an enzyme involved in glutathione biosynthesis) were significantly higher than in livers perfused with other bile salts. In ursodeoxycholate perfused livers, methionine adenosyltransferase showed a predominant tetrameric conformation which is the isoform with highest activity at physiological concentrations of substrate. In contrast, the dimeric form prevailed in livers perfused with taurocholate or deoxycholate. The hepatic activities of gamma-glutamylcysteine synthetase and gamma-glutamyltranspeptidase, enzymes involved, respectively, in biosynthetic and catabolic pathways of glutathione, were not modified by bile salts. CONCLUSIONS: Ursodeoxycholate specifically enhanced methionine adenosyltransferase activity and hepatic glutathione levels. As glutathione is a defensive substance against oxidative cell damage, our observations provide an additional explanation for the known hepatoprotective effects of ursodeoxycholate.
Permanent link: http://hdl.handle.net/10171/23283
Appears in Collections:DA - CIMA - Terapia génica y Hepatología - Hepatología experimental - Artículos de revista

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