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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología clínica > DA - CIMA - Terapia génica y Hepatología - Inmunología clínica - Artículos de revista >

Expression of interferon-alpha subtypes in peripheral mononuclear cells from patients with chronic hepatitis C: a role for interferon-alpha5
Authors: Larrea, E. (Esther)
Alberdi, A. (A.)
Castelruiz, Y. (Yurdana)
Boya, P. (Patricia)
Civeira, M.P. (María Pilar)
Prieto, J. (Jesús)
Keywords: Hepatitis C virus
Interferon regulatory factor
Interferon-a subtypes
Peripheral blood mononuclear cells
Sendai virus
Issue Date: 2001
Publisher: Blackwell Publishing
Publisher version: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2893.2001.00273.x/abstract
ISSN: 1365-2893
Citation: Larrea E, Alberdi A, Castelruiz Y, Boya P, Civeira MP, Prieto J. Expression of interferon-alpha subtypes in peripheral mononuclear cells from patients with chronic hepatitis C: a role for interferon-alpha5. J Viral Hepat 2001 Mar;8(2):103-110.
Abstract
Interferon (IFN)-alpha is a family of antiviral proteins encoded by different genes. The biological significance of the existence of various IFN-alpha subtypes is not clear. We have investigated the interferon system in chronic hepatitis C virus (HCV) infection, a disease that responds to interferon-alpha2 therapy in only a limited proportion of cases. We analysed the expression of interferon regulatory factor (IRF)-1, IRF-2, and IFN-alpha subtypes in nonstimulated and Sendai virus-stimulated peripheral blood mononuclear cells (PBMC) from HCV infected patients and healthy controls. We observed that the IRF-1 mRNA and IRF-1/IRF-2 ratios were increased in PBMC from hepatitis C patients with respect to normal subjects. Sendai virus stimulation of PBMC led to a significant increase in the levels of IRF-1, IRF-2 and IFN-alpha mRNAs and in the production of IFN-alpha protein with respect to basal values in healthy controls as well as in patients with HCV infection. In addition, we found that while natural HCV infection induced increased IFN-alpha5 expression in PBMC, in vitro infection of these cells with Sendai virus caused a raise in the expression of IFN-alpha8 in both patients and normal controls. In summary, our results indicate that virus-induced activation of the IFN system in human PBMC is associated with selective expression of individual IFN-alpha subtypes, IFN-alpha5 being the specific subtype induced in PBMC from patients with chronic HCV infection.
Permanent link: http://hdl.handle.net/10171/23330
Appears in Collections:DA - CUN - Medicina interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología clínica - Artículos de revista

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