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Please use this identifier to cite or link to this item:
http://hdl.handle.net/10171/23775
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| Title: | Screening of the human tumor necrosis factor-alpha (TNF-α) gene promoter polymorphisms by PCR–DGGE analysis |
| Author(s) : | Patiño, A.(Ana)
Sotillo, E. (Elena)
Modesto, C. (C.)
Sierrasesumaga, L. (Luis) |
| Issue Date: | 1999 |
| Publisher: | Elsevier |
| Citation: | Patiño-García A, Sotillo-Piñeiro E, Modesto C, Sierrasesúmaga L. Screening of the human tumor necrosis factor-alpha (TNF-alpha) gene promoter polymorphisms by PCR-DGGE analysis. Mutat Res. 1999 Aug;406(2-4):121-5. |
| Keywords: | Polymerase Chain Reaction/methods
Polymorphism, Restriction Fragment Length
Tumor Necrosis Factor-alpha/genetics |
| Abstract: | We have designed a new PCR-DGGE technique that enables detection of base changes in the TNF-alpha gene promoter. Screening of 130 samples from Spanish children has shown that this technique accurately detects the altered band patterns induced by the presence of the polymorphisms at positions -376, -308, -238 and -163 of the promoter sequence. Although further analysis are needed to fully characterise the alterations detected, we believe that this PCR-DGGE technique is a rapid and sensitive first approach to the genetic characterisation of the TNF-alpha promoter |
| URI: | http://hdl.handle.net/10171/23775 |
| Publisher version (URL): | http://dx.doi.org/10.1016/S1383-5726(99)00004-7 |
| Appears in Collections: | DA - CUN - Pediatría - Artículos de revista
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