Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Navarro, G. (Gemma) | - |
dc.creator | Sawant, R.R. (Rupa R.) | - |
dc.creator | Biswas, S. (Swati) | - |
dc.creator | Essex, S. (Sean) | - |
dc.creator | Tros-de-Ilarduya, C. (Conchita) | - |
dc.creator | Torchilin, V.P. (Vladimir P.) | - |
dc.date.accessioned | 2014-09-17T16:23:11Z | - |
dc.date.available | 2014-09-17T16:23:11Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Navarro G, Sawant RR, Biswas S, Essex S, Tros-de-Ilarduya C, Torchilin VP. P-glycoprotein silencing with siRNA delivered by DOPEmodified PEI overcomes doxorubicin resistance in breast cancer cells. Nanomedicine 2012 Jan;7(1):65–78 | es_ES |
dc.identifier.issn | 1743-5889 | - |
dc.identifier.uri | https://hdl.handle.net/10171/36627 | - |
dc.description.abstract | AIMS: Multidrug resistance (MDR) mediated by overexpression of drug efflux transporters such as P-glycoprotein (P-gp), is a major problem, limiting successful chemotherapy of breast cancer. The use of siRNA to inhibit P-gp expression in MDR tumors is an attractive strategy to improve the effectiveness of anticancer drugs. METHOD: We have synthesized a novel conjugate between a phospholipid (dioleoylphosphatidylethanolamine) and polyethylenimine (PEI) for siRNA delivery, for the purpose of silencing P-gp to overcome doxorubicin resistance in MCF-7 human breast cancer cells. RESULTS: The dioleoylphosphatidylethanolamine-PEI conjugate enhanced the transfection efficacy of low-molecular-weight PEI, which was otherwise totally ineffective. In addition, the polyethylene glycol/lipid coating of the new complexes gave rise to small micelle-like nanoparticles with improved biocompatibility properties. Both coated and noncoated formulations delivered P-gp-specific siRNA to MDR cells. DISCUSSION: The combination of doxorubicin and P-gp silencing formulations led to a twofold increase of doxorubicin uptake and a significant improvement of the therapeutic effect of doxorubicin in resistant cells. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Future Medicine | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | MCF-7 breast cancer cell | es_ES |
dc.subject | P-glycoprotein | es_ES |
dc.subject | Polyethylenimine | es_ES |
dc.subject | SiRNA delivery | es_ES |
dc.subject | Micelle-like nanoparticle | es_ES |
dc.subject | Multidrug resistance | es_ES |
dc.title | P-glycoprotein silencing with siRNA delivered by DOPEmodified PEI overcomes doxorubicin resistance in breast cancer cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.2217/nnm.11.93 | es_ES |
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